Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.62 extracted from

  • Kobayashi, T.; Suzuki, K.
    The glycosylceramidase in the murine intestine. Purification and substrate specificity (1981), J. Biol. Chem., 256, 7768-7773.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
taurodeoxycholate activation Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
glucosylceramide
-
Mus musculus
0.065
-
galactosylceramide
-
Mus musculus
0.18
-
phlorizin
-
Mus musculus
0.24
-
galactosylsphingosine
-
Mus musculus
0.24
-
4-methylumbelliferyl-beta-D-galactoside
-
Mus musculus
0.27
-
lactosylceramide
-
Mus musculus
0.36
-
4-nitrophenyl-beta-D-fucoside
-
Mus musculus
0.37
-
glucosylsphingosine
-
Mus musculus
0.45
-
4-methylumbelliferyl-alpha-L-arabinoside
-
Mus musculus
0.53
-
4-methylumbelliferyl-beta-D-glucoside
-
Mus musculus
1.3
-
4-O-beta-D-glucopyranosyl-D-glucopyranoside
-
Mus musculus
1.4
-
4-methylumbelliferyl-beta-D-xyloside
-
Mus musculus
2.78
-
4-O-beta-D-glucopyranosyl-D-glucopyranoside
-
Mus musculus
4.8
-
4-O-beta-D-galactopyranosyl-D-glucosamine
-
Mus musculus
22
-
6-O-beta-D-glucopyranosyl-D-glucopyranoside
-
Mus musculus
28
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
Mus musculus
67
-
4-O-beta-D-galactopyranosyl-D-fructoside
-
Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
290000 300000 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-fructoside + H2O Mus musculus very low activity beta-D-galactose + D-fructose
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Mus musculus best substrate D-galactose + beta-D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-N-acetylglucosamine + H2O Mus musculus poor activity beta-D-galactose + N-acetylglucosamine
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Mus musculus cellobiose beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Mus musculus low activity beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Mus musculus gentiobiose beta-D-glucose + D-glucose
-
?
galactosylceramide + H2O Mus musculus
-
beta-D-galactose + ceramide
-
ir
galactosylsphingosine + H2O Mus musculus very low activity beta-D-galactose + sphingosine
-
?
glucosylceramide + H2O Mus musculus
-
beta-D-glucose + ceramide
-
?
glucosylsphingosine + H2O Mus musculus very low activity beta-D-glucose + N-acylsphingosine
-
?
lactosylceramide + H2O Mus musculus low activity beta-D-glucose + galactosylceramide
-
?
phlorizin + H2O Mus musculus low activity phloretin + beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
intestine small intestine Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.78
-
galactosylceramide hydrolysis Mus musculus
2.67
-
glucosylceramide hydrolysis Mus musculus
15.33
-
lactose hydrolysis Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-L-arabinoside + H2O
-
Mus musculus 4-methylumbelliferone + alpha-L-arabinose
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
-
Mus musculus 4-methylumbelliferone + beta-D-galactose
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
-
Mus musculus 4-methylumbelliferone + beta-D-glucose
-
?
4-methylumbelliferyl-beta-D-xyloside + H2O low activity Mus musculus 4-methylumbelliferone + beta-D-xylose
-
?
4-nitrophenyl beta-D-fucoside + H2O
-
Mus musculus 4-nitrophenol + beta-D-fucose
-
?
4-O-beta-D-galactopyranosyl-D-fructoside + H2O very low activity Mus musculus beta-D-galactose + D-fructose
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O best substrate Mus musculus D-galactose + beta-D-glucose
-
?
4-O-beta-D-galactopyranosyl-D-glucosamine + H2O
-
Mus musculus ?
-
?
4-O-beta-D-galactopyranosyl-D-N-acetylglucosamine + H2O poor activity Mus musculus beta-D-galactose + N-acetylglucosamine
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Mus musculus beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O
-
Mus musculus ?
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O low activity Mus musculus beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Mus musculus beta-D-glucose + D-glucose
-
?
galactosylceramide + H2O
-
Mus musculus beta-D-galactose + ceramide
-
?
galactosylceramide + H2O
-
Mus musculus beta-D-galactose + ceramide
-
ir
galactosylsphingosine + H2O very low activity Mus musculus beta-D-galactose + sphingosine
-
?
glucosylceramide + H2O
-
Mus musculus beta-D-glucose + ceramide
-
?
glucosylsphingosine + H2O very low activity Mus musculus beta-D-glucose + N-acylsphingosine
-
?
lactosylceramide + H2O low activity Mus musculus beta-D-glucose + galactosylceramide
-
?
phlorizin + H2O low activity Mus musculus phloretin + beta-D-glucose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
more than 90% of activity lost within 20 min Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
hydrolysis of galactosyl- or glucosylceramide in the presence of taurodeoxycholate Mus musculus
5.5 5.7 hydrolysis of disaccharides or artificial substrates Mus musculus