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Literature summary for 3.2.1.62 extracted from

  • Skovbjerg, H.; Sjöström, H.; Noren, O.
    Purification and characterization of amphiphilic lactase/phlorizin hydrolase from human small intestine (1981), Eur. J. Biochem., 114, 653-661.
    View publication on PubMed
Search for more literature for 3.2.1.62

General Stability

General Stability Organism
sensitive to repeated freezing and thawing Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
phlorizin competitive inhibition of lactase activity; Ki: 0.44 mM Homo sapiens
Tris(hydroxymethyl)aminomethane competitive inhibition of lactase acitiviy, phlorizin activity not affected, Ki: 12 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 phlorizin
-
 Homo sapiens
4.4
-
 4-O-beta-D-glucopyranosyl-D-glucospyranoside
-
 Homo sapiens
21
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microvillus
-
 Homo sapiens 5902
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 2 * 160000, SDS-PAGE Homo sapiens
280000
-
 gel filtration, hydrophilic form Homo sapiens
320000
-
 gel filtration, amphiphilic form Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Homo sapiens best substrate D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens cellobiose beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens gentiobiose beta-D-glucose + D-glucose
-
 ?
phlorizin + H2O Homo sapiens
-
 phloretin + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, immunoadsorbent chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
intestine small intestine Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Homo sapiens 2-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O low activity Homo sapiens 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + beta-D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O best substrate Homo sapiens D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Homo sapiens beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Homo sapiens beta-D-glucose + D-glucose
-
 ?
phlorizin + H2O
-
 Homo sapiens phloretin + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 160000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 no detectable loss of activity for several weeks Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2 9 little activity outside this range Homo sapiens