Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',4'-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside | rapidly inhibits phlorizin hydrolase activity by covalently binding to the glutamic acid residue of the active site, lactase activity more slowly inhibited, pre-incubation with glucal protects the lactase active site from inactivation | Ovis aries aries | |
2-fluoro-dinitrophenylglucoside | specifically inhibits phlorizin hydrolase, lactase activity is inhibited slowly | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
phlorizin | - |
Ovis aries aries | |
0.002 | - |
phlorizin | pH 6, 37°C | Ovis aries | |
0.044 | - |
quercetin-4-glucopyranoside | - |
Ovis aries aries | |
0.044 | - |
quercetin-4'-glucoside | pH 6, 37°C | Ovis aries | |
0.046 | - |
quercetin 3-O-beta-D-glucopyranoside | - |
Ovis aries aries | |
0.046 | - |
quercetin-3-glucoside | pH 6, 37°C | Ovis aries | |
0.085 | - |
genistein-7-glucopyranoside | - |
Ovis aries aries | |
0.085 | - |
genistein-7-glucoside | pH 6, 37°C | Ovis aries | |
0.149 | - |
daidzein-7-glucopyranoside | - |
Ovis aries aries | |
0.149 | - |
daidzein-7-glucoside | pH 6, 37°C | Ovis aries | |
8.8 | - |
4-O-beta-D-galactopyranosyl-D-glucopyranoside | - |
Ovis aries aries | |
8.8 | - |
lactose | pH 6, 37°C | Ovis aries |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
brush border | luminal site | Ovis aries aries | 5903 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Ovis aries aries | hydrolysis located on a specific active site for lactose hydrolysis | D-galactose + beta-D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Ovis aries aries | acts on dietary glycosides before absorption | D-galactose + beta-D-glucose | - |
? | |
phlorizin + H2O | Ovis aries aries | hydrolysis located on a specific active site for phlorizin hydrolysis | phloretin + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Ovis aries aries | - |
sheep | - |
Purification (Comment) | Organism |
---|---|
to homogeneity | Ovis aries aries |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a beta-D-glucosyl-N-acylsphingosine + H2O = a ceramide + beta-D-glucose | mechanism | Ovis aries |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | small intestine | Ovis aries aries | - |
small intestine | - |
Ovis aries | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | hydrolysis located on a specific active site for lactose hydrolysis | Ovis aries aries | D-galactose + beta-D-glucose | - |
? | |
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | acts on dietary glycosides before absorption | Ovis aries aries | D-galactose + beta-D-glucose | - |
? | |
daidzein 7-O-beta-D-glucopyranoside + H2O | - |
Ovis aries aries | daidzein + beta-D-glucose | - |
? | |
daidzin + H2O | - |
Ovis aries | 3-(4-hydroxyphenyl)-7-hydroxy-chromen-4-one + D-glucose | - |
? | |
genistein-7-glucopyranoside + H2O | - |
Ovis aries aries | genistein + beta-D-glucose | - |
? | |
genistein-7-glucoside + H2O | - |
Ovis aries | genistein + D-glucose | - |
? | |
lactose + H2O | reaction is not inhibited by 2-fluoro-dinitrophenylglucoside | Ovis aries | D-galactose + glucose | - |
? | |
additional information | no substrate: rutin, naringin | Ovis aries | ? | - |
? | |
phlorizin + H2O | hydrolysis located on a specific active site for phlorizin hydrolysis | Ovis aries aries | phloretin + beta-D-glucose | - |
? | |
phlorizin + H2O | reaction is inhibited by 2-fluoro-dinitrophenylglucoside | Ovis aries | ? | - |
? | |
quercetin 3-O-beta-D-glucopyranoside + H2O | - |
Ovis aries aries | quercetin + beta-D-glucose | - |
? | |
quercetin 4'-O-beta-D-glucopyranoside + H2O | flavone glucosides more efficiently hydrolysed than isoflavone glucosides, major contribution to the hydrolysis is from the lactase site | Ovis aries aries | quercetin + beta-D-glucose | - |
? | |
quercetin-3-glucoside + H2O | reaction is marginally inhibited by 2-fluoro-dinitrophenylglucoside | Ovis aries | quercetin + D-glucose | - |
? | |
quercetin-4'-glucoside + H2O | reaction is marginally inhibited by 2-fluoro-dinitrophenylglucoside | Ovis aries | quercetin + D-glucose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
phlorizin | - |
Ovis aries aries | |
0.5 | - |
phlorizin | pH 6, 37°C | Ovis aries | |
2.1 | - |
daidzein-7-beta-D-glucopyranoside | - |
Ovis aries aries | |
2.1 | - |
daidzein-7-glucoside | pH 6, 37°C | Ovis aries | |
6.3 | - |
quercetin 3-O-beta-D-glucopyranoside | - |
Ovis aries aries | |
6.3 | - |
quercetin-3-glucoside | pH 6, 37°C | Ovis aries | |
6.5 | - |
genistein-7-beta-D-glucopyranoside | - |
Ovis aries aries | |
6.5 | - |
genistein-7-glucoside | pH 6, 37°C | Ovis aries | |
7.5 | - |
quercetin-4-beta-D-glucopyranoside | - |
Ovis aries aries | |
7.5 | - |
quercetin-4'-glucoside | pH 6, 37°C | Ovis aries | |
33.8 | - |
4-O-beta-D-galactopyranosyl-D-glucopyranoside | - |
Ovis aries aries | |
33.8 | - |
lactose | pH 6, 37°C | Ovis aries |