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Literature summary for 3.2.1.60 extracted from

  • Kim, T.U.; Gu, B.G.; Jeong, J.Y.; Byun, S.M.; Shin, Y.C.
    Purification and characterization of a maltotetraose-forming alkaline alpha-amylase from an alkalophilic Bacillus strain, GM8901 (1995), Appl. Environ. Microbiol., 61, 3105-3112.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 82% inhibition Bacillus sp. (in: Bacteria)
Hg2+ 1 mM HgCl2, complete inhibition Bacillus sp. (in: Bacteria)
phenylmethylsulfonyl fluoride 1 mM, 4°C, 10 h incubation, 76% inhibition Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 1 mM AgNO3 activates Bacillus sp. (in: Bacteria)
Ca2+ 1 mM CaCl2 activates Bacillus sp. (in: Bacteria)
Co2+ 1 mM CoCl2 activates Bacillus sp. (in: Bacteria)
Cu2+ 1 mM CuCl2 activates Bacillus sp. (in: Bacteria)
Mg2+ 1 mM MgCl2 activates Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus sp. (in: Bacteria) five alkaline amylases are produced in culture broth ?
-
?
additional information Bacillus sp. (in: Bacteria) GM8901 five alkaline amylases are produced in culture broth ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
GM8901
-
Bacillus sp. (in: Bacteria) GM8901
-
GM8901
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Reaction

Reaction Comment Organism Reaction ID
(Glcalpha(1-4))n + H2O = (Glcalpha(1-4))n-4 + (Glcalpha(1-4))4 exo-cleavage mechanism Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
158
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Bacillus sp. (in: Bacteria) maltotetraose + ?
-
?
amylose + H2O
-
Bacillus sp. (in: Bacteria) maltotetraose
-
?
amylose + H2O
-
Bacillus sp. (in: Bacteria) GM8901 maltotetraose
-
?
maltoheptaose + H2O
-
Bacillus sp. (in: Bacteria) maltotetraose + maltotriose
-
?
maltohexaose + H2O
-
Bacillus sp. (in: Bacteria) maltotetraose + maltose
-
?
maltohexaose + H2O
-
Bacillus sp. (in: Bacteria) GM8901 maltotetraose + maltose
-
?
maltopentaose + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
maltopentaose + H2O
-
Bacillus sp. (in: Bacteria) GM8901 ?
-
?
additional information five alkaline amylases are produced in culture broth Bacillus sp. (in: Bacteria) ?
-
?
additional information five alkaline amylases are produced in culture broth Bacillus sp. (in: Bacteria) GM8901 ?
-
?
starch + H2O
-
Bacillus sp. (in: Bacteria) maltotetraose
-
?
starch + H2O
-
Bacillus sp. (in: Bacteria) GM8901 maltotetraose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C, 40% of maximal activity, 80°C, 20% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
1 mM CaCl2 and 1% soluble starch enhance thermostability Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5 12
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
6 13 pH 6.0, 30%, pH 13.0, 55% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 13 stable between, 85-100% of maximal activity Bacillus sp. (in: Bacteria)