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Literature summary for 3.2.1.60 extracted from
- Purification and characterization of two forms of maltotetraose-forming amylase from Pseudomonas stutzeri (1990), Agric. Biol. Chem., 54, 737-743.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Pseudomonas stutzeri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
G4-2 form, SDS-PAGE | Pseudomonas stutzeri |
| 57000 | - |
G4-1 form, SDS-PAGE | Pseudomonas stutzeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
MO-19 | - |
| Pseudomonas stutzeri MO-19 | - |
MO-19 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two active forms, G4-1 and G4-2 | Pseudomonas stutzeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 313 | - |
G4-1 form | Pseudomonas stutzeri |
| 364 | - |
G4-2 form | Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Pseudomonas stutzeri | maltotetraose + ? | - |
? |  |
| amylopectin + H2O | - |
Pseudomonas stutzeri MO-19 | maltotetraose + ? | - |
? | |
| glycogen + H2O | - |
Pseudomonas stutzeri | maltotetraose | - |
? | |
| glycogen + H2O | - |
Pseudomonas stutzeri MO-19 | maltotetraose | - |
? | |
| starch + H2O | - |
Pseudomonas stutzeri | maltotetraose | - |
? | |
| starch + H2O | - |
Pseudomonas stutzeri MO-19 | maltotetraose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
G4-1 and G4-2 form | Pseudomonas stutzeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
G4-1-form heat-sensitive than G4-2 form | Pseudomonas stutzeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
G4-1 and G4-2 form | Pseudomonas stutzeri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | G4-1 and G4-2 form, stable between | Pseudomonas stutzeri |
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