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Literature summary for 3.2.1.59 extracted from

  • Cherdvorapong, V.; Fujiki, H.; Suyotha, W.; Takeda, Y.; Yano, S.; Takagi, K.; Wakayama, M.
    Enzymatic and molecular characterization of alpha-1,3-glucanase (AglST2) from Streptomyces thermodiastaticus HF3-3 and its relation with alpha-1,3-glucanase HF65 (AglST1) (2018), J. Gen. Appl. Microbiol., 65, 18-25 .
    View publication on PubMed
Search for more literature for 3.2.1.59

Activating Compound

Activating Compound Comment Organism Structure
NaCl the enzyme activity is significantly stimulated in the presence of 5-20% (w/v) NaCl (140% activity at 15% (w/v)) Streptomyces thermodiastaticus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces thermodiastaticus

General Stability

General Stability Organism
the enzyme retains full activity when treated with 20% (w/v) NaCl for 1 h Streptomyces thermodiastaticus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 57% residual activity at 1 mM Streptomyces thermodiastaticus
Cu2+ 44% residual activity at 1 mM Streptomyces thermodiastaticus
EDTA 1-50 mM EDTA decreases the relative activity by almost 50% Streptomyces thermodiastaticus
Fe2+ 55% residual activity at 1 mM Streptomyces thermodiastaticus
additional information not inhibited by dithiothreitol Streptomyces thermodiastaticus
Ni2+ 64% residual activity at 1 mM Streptomyces thermodiastaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 140% activity at 1 mM Streptomyces thermodiastaticus
Mn2+ 140% activity at 1 mM Streptomyces thermodiastaticus

Organism

Organism UniProt Comment Textmining
Streptomyces thermodiastaticus
-
-
-
Streptomyces thermodiastaticus HF3-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellufine A500 column chromatography and HiTrap Q column chromatography Streptomyces thermodiastaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,3-glucan + H2O 100% activity Streptomyces thermodiastaticus ?
-
 ?
alpha-1,3-glucan + H2O 100% activity Streptomyces thermodiastaticus HF3-3 ?
-
 ?
additional information no activity with cellulose, xylan, dextran, amylopectin, laminarin, and amylose Streptomyces thermodiastaticus ?
-
 ?
additional information no activity with cellulose, xylan, dextran, amylopectin, laminarin, and amylose Streptomyces thermodiastaticus HF3-3 ?
-
 ?
nigeran + H2O 10% activity compared to alpha-1,3-glucan Streptomyces thermodiastaticus ?
-
 ?
nigeran + H2O 10% activity compared to alpha-1,3-glucan Streptomyces thermodiastaticus HF3-3 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 91000, SDS-PAGE Streptomyces thermodiastaticus
? x * 76860, calculated from amino acid sequence Streptomyces thermodiastaticus

Synonyms

Synonyms Comment Organism
AglST2
-
 Streptomyces thermodiastaticus
alpha-1,3-glucanase HF90
-
 Streptomyces thermodiastaticus
mutanase
-
 Streptomyces thermodiastaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces thermodiastaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the residual activity of the enzyme is more than 80% after incubation at 65°C for 1 h Streptomyces thermodiastaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Streptomyces thermodiastaticus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 the enzyme retains 80% of the original activity over a pH range of 5.0-7.0, however, the activity gradually decreases at a pH above 7.5 Streptomyces thermodiastaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 the residual activity of the enzyme is more than 80% after incubation at pH 4.5-7.0 for 1 h Streptomyces thermodiastaticus