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Literature summary for 3.2.1.59 extracted from

  • Suyotha, W.; Fujiki, H.; Cherdvorapong, V.; Takagi, K.; Yano, S.; Wakayama, M.
    A novel thermostable alpha-1,3-glucanase from Streptomyces thermodiastaticus HF 3-3 (2017), J. Gen. Appl. Microbiol., 63, 296-304 .
    View publication on PubMed
Search for more literature for 3.2.1.59

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 124% activity at 1 mM Streptomyces thermodiastaticus

General Stability

General Stability Organism
the enzyme is stable in the presence of chemicals like SDS, benzethonium chloride, and sodium fluoride at a concentration of 1% (w/v). The enzyme also exhibits salt tolerance at a concentration up to 20% (w/v) Streptomyces thermodiastaticus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ less than 20% residual activity at 1 mM Streptomyces thermodiastaticus
Cu2+ 80% residual activity at 1 mM Streptomyces thermodiastaticus
EDTA about 80% residual activity at 1 mM Streptomyces thermodiastaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 125% activity at 1 mM Streptomyces thermodiastaticus
Co2+ 160% activity at 1 mM Streptomyces thermodiastaticus
Li+ 115% activity at 1 mM Streptomyces thermodiastaticus
Mn2+ 124% activity at 1 mM Streptomyces thermodiastaticus
additional information Na+, K+, NH4+, Mg2+, Fe2+, Zn2+, and Ni2+ do not significantly affect the enzyme activity Streptomyces thermodiastaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45700
-
 gel filtration Streptomyces thermodiastaticus

Organism

Organism UniProt Comment Textmining
Streptomyces thermodiastaticus
-
-
-
Streptomyces thermodiastaticus HF 3-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulofine column chromatography and butyl-Toyopearl 650M column chromatography Streptomyces thermodiastaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,3-glucan + H2O the enzyme exhibits high specificity against alpha-1,3-glucan Streptomyces thermodiastaticus ?
-
 ?
alpha-1,3-glucan + H2O the enzyme exhibits high specificity against alpha-1,3-glucan Streptomyces thermodiastaticus HF 3-3 ?
-
 ?
additional information no activity with dextran, cellulose, amylose, amylopectin, and laminarin Streptomyces thermodiastaticus ?
-
 ?
additional information no activity with dextran, cellulose, amylose, amylopectin, and laminarin Streptomyces thermodiastaticus HF 3-3 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Streptomyces thermodiastaticus

Synonyms

Synonyms Comment Organism
alpha-1,3-glucanase HF65
-
 Streptomyces thermodiastaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Streptomyces thermodiastaticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 more than 60% activity between 50 and 70°C Streptomyces thermodiastaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65 70 the purified enzyme retains more than 50% of maximum activity after incubation at 65°C more than 2 h and exhibits about 95% of full activity after incubation at 65°C for 10 min. After treatment at 70°C for 120 min, the enzyme shows 20% residual activity Streptomyces thermodiastaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Streptomyces thermodiastaticus

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 more than 60% activity between pH 4.0 and 6.5 Streptomyces thermodiastaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.5 the purified enzyme retains more than 80% of maximum activity after 2 h at pH 4.5-6.5 Streptomyces thermodiastaticus