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Literature summary for 3.2.1.59 extracted from

  • Suyotha, W.; Yano, S.; Takagi, K.; Rattanakit-Chandet, N.; Tachiki, T.; Wakayama, M.
    Domain structure and function of alpha-1,3-glucanase from Bacillus circulans KA-304, an enzyme essential for degrading basidiomycete cell walls (2013), Biosci. Biotechnol. Biochem., 77, 639-647.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and deletion mutant enzymes in Escherichia coli strain Rosetta-gami B (DE3), secretion of enzyme to the culture medium, recombinant expression as GFP-tagged proteins, overview Niallia circulans

Protein Variants

Protein Variants Comment Organism
additional information generation of domain deletion enzyme mutants lacking discoidin domains DS1 and DS2, the uncharacterized conserved domain, and/or carbohydrate binding module family 6, AglDELTADS1, AglDELTADS1CB6, AglDELTADS1CB6DS2, and AglDELTADCD-UCD. Only the DS1 domain deletion mutant shows some activity, the other mutants show only residual or no activity, overview Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km is 5.6 mg/ml for alpha-1,3-glucan with the recombinant wild-type enzyme, pH 6.5, 30°C, kinetics of recombinant wild-type and domain deletion mutants, overview Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans KA-304
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant secreted wild-type and deletion mutant enzymes from Escherichia coli strain Rosetta-gami B (DE3) culture supernatant by dialysis, anion exchange chromatography, ammonium sulfate fractionation and hydrophobic interaction chromatography Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,3-glucan + H2O prepared from sucrose using Streptococcus mutans ATCC700610 glucosyltransferase I Niallia circulans ?
-
?
alpha-1,3-glucan + H2O prepared from sucrose using Streptococcus mutans ATCC700610 glucosyltransferase I Niallia circulans KA-304 ?
-
?
fungal cell walls + H2O
-
Niallia circulans ?
-
?
fungal cell walls + H2O
-
Niallia circulans KA-304 ?
-
?

Subunits

Subunits Comment Organism
More the enzyme structure includes an N-terminal discoidin domain (DS1), a carbohydrate binding module family 6 (CB6), threonine and proline repeats (TPs), a second discoidin domain (DS2), an uncharacterized conserved domain (UCD), and a C-terminal catalytic domain Niallia circulans

Synonyms

Synonyms Comment Organism
Agl-KA
-
Niallia circulans
alpha-1,3-glucanase
-
Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information the amount of reducing sugar in the supernatant of the enzyme assay is determined Niallia circulans
91.3
-
alpha-1,3-glucan recombinant wild-type enzyme, pH 6.5, 30°C Niallia circulans
106.6
-
alpha-1,3-glucan recombinant mutant AglDELTADS1, pH 6.5, 30°C Niallia circulans
130.7
-
alpha-1,3-glucan recombinant mutant AglDELTADS1CB6, pH 6.5, 30°C Niallia circulans
134.4
-
alpha-1,3-glucan recombinant mutant AglDELTADS1CB6DS2, pH 6.5, 30°C Niallia circulans
158.7
-
alpha-1,3-glucan recombinant mutant AglDELTADCD-UCD, pH 6.5, 30°C Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Niallia circulans

General Information

General Information Comment Organism
malfunction domain deletion enzyme mutants lacking discoidin domains DS1 and DS2 and carbohydrate binding module family 6 domain exhibit lower alpha-1,3-glucan-hydrolyzing and substrate-binding activities than the wild-type enzyme. A dramatic decrease in protoplast formation in the Schizophyllum commune mycelium is observed given only a domain DS1 deletion Niallia circulans
additional information discoidin domains DS1 and DS2 and carbohydrate binding module family 6 domain are involved in alpha-1,3-glucan and fungal cell wall substrate binding, while the uncharacterized conserved domain is not, overview Niallia circulans
physiological function the enzyme is involved in fungal cell wall degradation Niallia circulans