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Literature summary for 3.2.1.59 extracted from
- Purification and properties of endo-1, 3-alpha-D-glucanase from Pseudomonas (1982), Biochim. Biophys. Acta, 715, 189-195.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Pseudomonas sp. |  |
| EDTA | - |
Pseudomonas sp. | |
| Fe3+ | - |
Pseudomonas sp. | |
| Hg2+ | - |
Pseudomonas sp. | |
| p-chloromercuribenzoic acid | - |
Pseudomonas sp. | |
| Pb2+ | - |
Pseudomonas sp. | |
| Zn2+ | - |
Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 80 | - |
limit glucan | - |
Pseudomonas sp. |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | increases activity | Pseudomonas sp. | |
| Co2+ | increases activity | Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 279000 | - |
aggregated form, gel filtration | Pseudomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| NRRL-B-12324 | Pseudomonas sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Pseudomonas sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 78.1 | - |
- |
Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 18 months, 17% loss of activity | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| limit glucan + H2O | - |
Pseudomonas sp. | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
- |
Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 5.5, 3 days, 3% loss of activity | Pseudomonas sp. |
| 60 | - |
loss of activity at or above | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Pseudomonas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | sharp decrease below pH 4.5, gradual decrease up to pH 8.0 | Pseudomonas sp. |
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