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Literature summary for 3.2.1.58 extracted from

  • Schwentke, J.; Sabel, A.; Petri, A.; Koenig, H.; Claus, H.
    The yeast Wickerhamomyces anomalus AS1 secretes a multifunctional exo-beta-1,3-glucanase with implications for winemaking (2014), Yeast, 31, 349-359.
    View publication on PubMed
Search for more literature for 3.2.1.58

Application

Application Comment Organism
food industry Exg2 is active under typical wine-related conditions, such as low pH (3.5–4.0), high sugar concentrations (up to 20% w/v), high ethanol concentrations (10–15% v/v), presence of sulfites (up to 2 mM) and various cations and may have multiple uses in wine making Wickerhamomyces anomalus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 100% of initial activity Wickerhamomyces anomalus
Cu2+ 1 mM, 94% of initial activity Wickerhamomyces anomalus
K+ 1 mM, 88% of initial activity Wickerhamomyces anomalus
Mg2+ 1 mM, 100% of initial activity Wickerhamomyces anomalus
Mn2+ 1 mM, 89% of initial activity Wickerhamomyces anomalus
additional information the enzyme is active under typical wine-related conditions, such as low pH (3.5-4.0), high sugar concentrations (up to 20% w/v), high ethanol concentrations (10-15% v/v), presence of sulfites (up to 2 mM) and various cations Wickerhamomyces anomalus
potassium metabisulfite 1 mM, 90% of initial activity Wickerhamomyces anomalus
Zn2+ 1 mM, 100% of initial activity Wickerhamomyces anomalus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Wickerhamomyces anomalus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47456
-
-
 Wickerhamomyces anomalus

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 10-15% v/v, about 70% of maximum actviity Wickerhamomyces anomalus

Organism

Organism UniProt Comment Textmining
Wickerhamomyces anomalus H6UWP3
-
-
Wickerhamomyces anomalus AS1 H6UWP3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arbutin + H2O
-
 Wickerhamomyces anomalus D-glucose + ?
-
 ?
arbutin + H2O
-
 Wickerhamomyces anomalus AS1 D-glucose + ?
-
 ?
cellobiose + H2O
-
 Wickerhamomyces anomalus 2 D-glucose
-
 ?
cellobiose + H2O
-
 Wickerhamomyces anomalus AS1 2 D-glucose
-
 ?
esculin + H2O
-
 Wickerhamomyces anomalus D-glucose + 4-hydroxyphenol
-
 ?
esculin + H2O
-
 Wickerhamomyces anomalus AS1 D-glucose + 4-hydroxyphenol
-
 ?
gentobiose + H2O
-
 Wickerhamomyces anomalus 2 D-glucose
-
 ?
gentobiose + H2O
-
 Wickerhamomyces anomalus AS1 2 D-glucose
-
 ?
laminarin + H2O
-
 Wickerhamomyces anomalus D-glucose + ? glucose is the sole hydrolysis product ?
laminarin + H2O
-
 Wickerhamomyces anomalus AS1 D-glucose + ? glucose is the sole hydrolysis product ?
polydatin + H2O
-
 Wickerhamomyces anomalus D-glucose + 3,4',5-trihydroxystilbene
-
 ?
salicin + H2O
-
 Wickerhamomyces anomalus D-glucose + 2-hydroxymethylphenol
-
 ?

Subunits

Subunits Comment Organism
? x * 47456, caluculated, mature protein Wickerhamomyces anomalus

Synonyms

Synonyms Comment Organism
EXG2
-
 Wickerhamomyces anomalus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Wickerhamomyces anomalus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Wickerhamomyces anomalus

pI Value

Organism Comment pI Value Maximum pI Value
Wickerhamomyces anomalus calculated
-
 4.8