Any feedback?
Literature summary for 3.2.1.58 extracted from
- Expression and characterization of a novel beta-glucosidase, with transglycosylation and exo-beta-1,3-glucanase activities, from Rhizomucor miehei (2015), Food Chem., 175, 431-438.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rhizomucor miehei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 50°C | Rhizomucor miehei |  |
| 0.92 | - |
cellotetraose | pH 5.0, 50°C | Rhizomucor miehei | |
| 1.26 | - |
laminaribiose | pH 5.0, 50°C | Rhizomucor miehei | |
| 1.5 | 2 | cellotriose | pH 5.0, 50°C | Rhizomucor miehei | |
| 2.29 | - |
laminaritriose | pH 5.0, 50°C | Rhizomucor miehei | |
| 2.32 | - |
Laminaran | pH 5.0, 50°C | Rhizomucor miehei | |
| 3.7 | - |
cellobiose | pH 5.0, 50°C | Rhizomucor miehei | |
| 4.15 | - |
gentobiose | pH 5.0, 50°C | Rhizomucor miehei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66400 | - |
gel filtration | Rhizomucor miehei |
| 76500 | - |
SDS-PAGE | Rhizomucor miehei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor miehei | A0A0B4RUW2 | cf. EC 3.2.1.21 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.1 | - |
substrate laminaran, pH 5.0, 50°C | Rhizomucor miehei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + D-glucose | 2.7% of the activity with 4-nitrophenyl alpha-D-glucopyranoside | ? | |
| 4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + D-xylose | 3.9% of the activity with 4-nitrophenyl alpha-D-glucopyranoside | ? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + D-glucose | - |
? | |
| cellobiose + H2O | - |
Rhizomucor miehei | 2 D-glucose | 62% of the activity with laminaribiose | ? | |
| cellotetraose + H2O | - |
Rhizomucor miehei | D-glucose + ? | 64% of the activity with laminaribiose | ? | |
| cellotriose + H2O | - |
Rhizomucor miehei | D-glucose + ? | 67% of the activity with laminaribiose | ? | |
| gentobiose + H2O | - |
Rhizomucor miehei | 2 D-glucose | 52% of the activity with laminaribiose | ? | |
| laminaran + H2O | 67% of the activity with laminaribiose | Rhizomucor miehei | D-glucose + gentiobiose + gentiotriose | - |
? | |
| laminaribiose + H2O | - |
Rhizomucor miehei | 2 D-glucose | - |
? | |
| laminaritriose + H2O | - |
Rhizomucor miehei | D-glucose + ? | 97% of the activity with laminaribiose | ? | |
| additional information | isoform Bglu3B exhibits a broad range of substrate specificity, catalyzing the cleavage of beta-1,2, beta-1,3, beta-1,4 and beta-1,6 linkages, in various oligosaccharides, to liberate glucose. Bglu3B shows relatively high activity toward laminaran and transglycosylation activity, enabling gentiobiose production. No substrates: 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, lactose, barley beta-glucan, Avicel, carboxymethyl cellulose, lichenan, birchwood xylan and chitin | Rhizomucor miehei | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bglu3B | - |
Rhizomucor miehei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Rhizomucor miehei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
Laminaran | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.043 | - |
cellobiose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.056 | - |
gentobiose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.059 | - |
cellotetraose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.067 | - |
cellotriose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.071 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.077 | - |
laminaritriose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.078 | - |
laminaribiose | pH 5.0, 50°C | Rhizomucor miehei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Rhizomucor miehei |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
cellobiose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.013 | - |
gentobiose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.014 | - |
Laminaran | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.033 | - |
laminaritriose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.044 | - |
cellotriose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.062 | - |
laminaribiose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.063 | - |
cellotetraose | pH 5.0, 50°C | Rhizomucor miehei | |
| 0.42 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 50°C | Rhizomucor miehei | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
