Any feedback?
Literature summary for 3.2.1.58 extracted from
- Expression and characterization of a novel antifungal exo-beta-1,3-glucanase from Chaetomium cupreum (2017), Appl. Biochem. Biotechnol., 182, 261-275.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Arcopilus cupreus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM, 35% residual activity | Arcopilus cupreus |  |
| Co2+ | 5 mM, 70% residual activity | Arcopilus cupreus | |
| Cu2+ | 5 mM, 63% residual activity | Arcopilus cupreus | |
| Hg2+ | 5 mM, 40% residual activity | Arcopilus cupreus | |
| Mg2+ | 5 mM, 90% residual activity | Arcopilus cupreus | |
| Mn2+ | 5 mM, 43% residual activity | Arcopilus cupreus | |
| additional information | actvitiy is not affected by 5 mM K+, Mg2+, Ca2+, or Zn2+ | Arcopilus cupreus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | Km value 2.84 mg/ml, pH 5.0, 45°C | Arcopilus cupreus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Arcopilus cupreus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arcopilus cupreus | - |
- |
- |
| Arcopilus cupreus Ame | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | 7.4% of the activity with laminarin | Arcopilus cupreus | D-glucose + ? | - |
? | |
| barley beta-glucan + H2O | 7.4% of the activity with laminarin | Arcopilus cupreus Ame | D-glucose + ? | - |
? | |
| laminaribiose + H2O | - |
Arcopilus cupreus | 2 D-glucose | complete hydrolysis within 2 h to yield glucose | ? | |
| laminaribiose + H2O | - |
Arcopilus cupreus Ame | 2 D-glucose | complete hydrolysis within 2 h to yield glucose | ? | |
| laminarin + H2O | - |
Arcopilus cupreus | D-glucose + ? | - |
? | |
| laminarin + H2O | - |
Arcopilus cupreus Ame | D-glucose + ? | - |
? | |
| laminaritriose + 2 H2O | - |
Arcopilus cupreus | 3 D-glucose | complete hydrolysis within 2 h to yield glucose | ? | |
| laminaritriose + 2 H2O | - |
Arcopilus cupreus Ame | 3 D-glucose | complete hydrolysis within 2 h to yield glucose | ? | |
| additional information | the enzyme exhibits high substrate specificity, almost exclusively catalyzing beta-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose. Additionally, it shows transglycosylation activity | Arcopilus cupreus | ? | - |
? | |
| additional information | the enzyme exhibits high substrate specificity, almost exclusively catalyzing beta-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose. Additionally, it shows transglycosylation activity | Arcopilus cupreus Ame | ? | - |
? | |
| zymosan A + H2O | 45% of the activity with laminarin | Arcopilus cupreus | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Arcopilus cupreus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glu17A | - |
Arcopilus cupreus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Arcopilus cupreus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Arcopilus cupreus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | purified Glu17A exhibits antifungal activity in vitro against pathogenic fungi. Glu17A exerts the strongest enzyme activities toward the cell walls of Fusarium commune, with a value of 2.1 U/mg | Arcopilus cupreus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
