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Literature summary for 3.2.1.58 extracted from

  • OConnell, E.; Piggott, C.; Tuohy, M.
    Purification of exo-1,3-beta-glucanase, a new extracellular glucanolytic enzyme from Talaromyces emersonii (2011), Appl. Microbiol. Biotechnol., 89, 685-696.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
glucono-delta-lactone
-
Rasamsonia emersonii
KMnO4 10 mM, 60% residual activity Rasamsonia emersonii
L-cysteine 10 mM, 68% residual activity Rasamsonia emersonii
N-bromosuccinimide 10 mM, 0.1% residual activity Rasamsonia emersonii
phenylglyoxal hydrate 10 mM, 86% residual activity Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
Laminaran KM-value is 1.66 mg/ml Rasamsonia emersonii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Rasamsonia emersonii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 10 mM, 82% residual activity Rasamsonia emersonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
PAGE Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii
-
member of glycosyl hydrolase family 5
-

Purification (Commentary)

Purification (Comment) Organism
-
Rasamsonia emersonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
592
-
pH 5.0, 50°C Rasamsonia emersonii

Storage Stability

Storage Stability Organism
4°C, pH 5.0, stable for at least 6 months Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminaran + H2O at pH 5.0, strictly specific for laminaran and laminarioligosaccharides, with a strict exo mode of action, with glucose being the sole reaction product even at the initial stages of hydrolysis Rasamsonia emersonii D-glucose + ?
-
?
laminaripentaose + H2O 118% of the activity with laminaran Rasamsonia emersonii D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
?
laminaritetraose + H2O 121% of the activity with laminaran Rasamsonia emersonii D-glucose + laminaribiose + laminaritriose
-
?
laminaritriose + H2O 115% of the activity with laminaran Rasamsonia emersonii D-glucose + laminaribiose
-
?
additional information no substrate: beta-1,4-glucans, beta-1,3/1,4-glucans or 4-nitrophenyl- and methylumbelliferyl-beta-D-glucopyranosides, carboxymethyl cellulose, avicel. Trace activity with pustulan Rasamsonia emersonii ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
half-life 18.3 h Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29550
-
Laminaran pH 5.0, 50°C Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
Rasamsonia emersonii

pH Range

pH Minimum pH Maximum Comment Organism
2.8 8 more than 50% of maximum activity within Rasamsonia emersonii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.25
-
glucono-delta-lactone pH 5.0, 50°C Rasamsonia emersonii

pI Value

Organism Comment pI Value Maximum pI Value
Rasamsonia emersonii isoelectric focusing
-
4.3