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Literature summary for 3.2.1.58 extracted from
- An extracellular Xu, Z.; Shih, M.C.; Poulton, J.E.: An extracellular exo-beta-(1,3)-glucanase from Pichia pastoris: purification, characterization, molecular cloning, and functional expression (2006), Protein Expr. Purif., 47, 118-127.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Komagataella pastoris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | apparent Km-value for laminarin is 3.5 mg/ml | Komagataella pastoris |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Komagataella pastoris | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45376 | - |
1 * 45376, calculated from sequence | Komagataella pastoris |
| 47000 | - |
1 * 47000, SDS-PAGE | Komagataella pastoris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Komagataella pastoris | - |
- |
- |
| Komagataella pastoris X-33 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the native enzyme is unglycosylated | Komagataella pastoris |
| proteolytic modification | the preproprotein is processed sequentially by signal peptidase and a Kex2-like endoprotease to yield a mature protein of 392 amino acids | Komagataella pastoris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Komagataella pastoris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.77 | - |
substrate: 4-nitrophenyl-beta-D-glucopyranoside | Komagataella pastoris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-xylopyranoside + H2O | 31% of the activity with 4-nitrophenyl-beta-D-glucopyranoside | Komagataella pastoris | 4-nitrophenol + D-xylose | - |
? |  |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | 31% of the activity with 4-nitrophenyl-beta-D-glucopyranoside | Komagataella pastoris X-33 | 4-nitrophenol + D-xylose | - |
? | |
| amygdalin + H2O | 82.2% of the activity with laminarin | Komagataella pastoris | ? | - |
? | |
| amygdalin + H2O | 82.2% of the activity with laminarin | Komagataella pastoris X-33 | ? | - |
? | |
| esculin + H2O | 24.2% of the activity with laminarin | Komagataella pastoris | ? | - |
? | |
| esculin + H2O | 24.2% of the activity with laminarin | Komagataella pastoris X-33 | ? | - |
? | |
| laminarin + H2O | - |
Komagataella pastoris | D-glucose + ? | - |
? | |
| laminarin + H2O | - |
Komagataella pastoris X-33 | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Komagataella pastoris |
| monomer | 1 * 45376, calculated from sequence | Komagataella pastoris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Exg1 | - |
Komagataella pastoris |
| exo-beta-(1,3)-glucanase | - |
Komagataella pastoris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Komagataella pastoris |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Komagataella pastoris | calculated from sequence | - |
4.5 |
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