Any feedback?
Literature summary for 3.2.1.58 extracted from
- Glucanases in Schizosaccharomyces. Isolation and properties of an exo-beta-glucanase from the cell extracts and culture fluid of Schizosaccharomyces japonicus var. Versatilis (1975), Biochim. Biophys. Acta, 401, 318-332.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-D-glucose | slight | Schizosaccharomyces japonicus |  |
| D-glucono-delta-lactone | Ki: 0.9 mM | Schizosaccharomyces japonicus | |
| Hg2+ | - |
Schizosaccharomyces japonicus | |
| indole-3-acetic acid | slight | Schizosaccharomyces japonicus | |
| Zn2+ | - |
Schizosaccharomyces japonicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pustulan 166 mg/ml | Schizosaccharomyces japonicus | |
| additional information | - |
additional information | laminarin 6.25 mg/ml | Schizosaccharomyces japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces japonicus | - |
var. versatilis | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 418.6fold | Schizosaccharomyces japonicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 180 | - |
- |
Schizosaccharomyces japonicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 1-4°C, sodium succinate buffer pH 5.0-5.5, 3 months, no loss of activity | Schizosaccharomyces japonicus |
| freeze drying, 50% activity loss | Schizosaccharomyces japonicus |
| frozen, 8 months, 50% activity loss | Schizosaccharomyces japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gentiobiose + H2O | - |
Schizosaccharomyces japonicus | D-glucose | - |
? | |
| laminarin + H2O | - |
Schizosaccharomyces japonicus | D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose | different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose | ? | |
| p-nitrophenyl-beta-D-glucoside | - |
Schizosaccharomyces japonicus | D-glucose + nitrophenol + glucan | - |
? | |
| pachyman + H2O | - |
Schizosaccharomyces japonicus | D-glucose | - |
? | |
| pustulan + H2O | - |
Schizosaccharomyces japonicus | D-glucose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
up to 50°C | Schizosaccharomyces japonicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Schizosaccharomyces japonicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
D-glucono-delta-lactone | - |
Schizosaccharomyces japonicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
