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Literature summary for 3.2.1.58 extracted from
- Comparative studies on beta-glucan hydrolases. Isolation and characterization of an exo(1 yields 3)-beta-glucanase from the snail, Helix pomatia (1975), Arch. Biochem. Biophys., 167, 165-175.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | - |
Helix pomatia |  |
| Ammonium molybdate | - |
Helix pomatia | |
| CuSO4 | - |
Helix pomatia | |
| D-glucono-delta-lactone | - |
Helix pomatia | |
| FeCl2 | - |
Helix pomatia | |
| HgCl2 | - |
Helix pomatia | |
| KCN | - |
Helix pomatia | |
| N-bromosuccinimide | - |
Helix pomatia | |
| Phenyl mercuric nitrate | - |
Helix pomatia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | lichenin 2.22 mg/ml | Helix pomatia | |
| additional information | - |
additional information | laminarin 1.22 mg/ml | Helix pomatia |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
x * 82000, SDS-PAGE | Helix pomatia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helix pomatia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Helix pomatia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24.2 | - |
- |
Helix pomatia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| room temperature, several hours | Helix pomatia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellodextrin + H2O | - |
Helix pomatia | D-glucose | - |
? | |
| CM-cellulose + H2O | very weak hydrolysis | Helix pomatia | D-glucose | - |
? | |
| gentiobiose + H2O | - |
Helix pomatia | D-glucose | - |
? | |
| glucan + H2O | barley beta-glucan | Helix pomatia | D-glucose + gentiobiose | D-glucose only | ? | |
| glucan + H2O | yeast glucan | Helix pomatia | D-glucose + gentiobiose | D-glucose only | ? | |
| glucan + H2O | oat beta-glucan | Helix pomatia | D-glucose + gentiobiose | D-glucose only | ? | |
| laminaribiose + H2O | - |
Helix pomatia | D-glucose | - |
? | |
| laminarin + H2O | - |
Helix pomatia | D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose | different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose | ir | |
| laminaripentaose + H2O | - |
Helix pomatia | D-glucose + laminaribiose + laminaritriose + laminaritetraose | different combinations and amount of these products, depending on organism | ? | |
| laminaritetraose + H2O | - |
Helix pomatia | D-glucose + laminaribiose + laminaritriose | different combinations and amount of these products, depending on organism | ? | |
| laminaritriose + H2O | - |
Helix pomatia | D-glucose + laminaribiose | D-glucose only | ? | |
| lichenin + H2O | - |
Helix pomatia | D-glucose | - |
? | |
| luteose + H2O | - |
Helix pomatia | D-glucose | - |
? | |
| pachyman + H2O | - |
Helix pomatia | D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 82000, SDS-PAGE | Helix pomatia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
- |
Helix pomatia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
up to 50°C | Helix pomatia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | 4.5 | - |
Helix pomatia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6.5 | - |
Helix pomatia |
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