Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
2 * 95000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
200000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
likely belonging to Bacillus naganoensis | - |
Bacillus sp. (in: Bacteria) US149 | - |
likely belonging to Bacillus naganoensis | - |
Purification (Comment) | Organism |
---|---|
3fold | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.6 | - |
pH 5, 60°C | Bacillus sp. (in: Bacteria) |
Storage Stability | Organism |
---|---|
4°C, no major loss of activity | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: amylose, maltoheptaose, starch, amylopectin | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | not: amylose, maltoheptaose, starch, amylopectin | Bacillus sp. (in: Bacteria) US149 | ? | - |
? | |
pullulan + H2O | IPU degrades alpha-1,4-glycosidic linkage from the reducing end adjacent to alpha-1,6-glycosidic linkage in pullulan | Bacillus sp. (in: Bacteria) | isopanose | - |
? | |
pullulan + H2O | IPU degrades alpha-1,4-glycosidic linkage from the reducing end adjacent to alpha-1,6-glycosidic linkage in pullulan | Bacillus sp. (in: Bacteria) US149 | isopanose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 95000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
IPU | - |
Bacillus sp. (in: Bacteria) |
More | classified under pullulanase type II | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
calcium does not increase the thermostability | Bacillus sp. (in: Bacteria) |
60 | - |
60 min, 16% loss of activity | Bacillus sp. (in: Bacteria) |
70 | - |
60 min, 30% loss of activity | Bacillus sp. (in: Bacteria) |
80 | - |
pH 5, half-life: 48 min | Bacillus sp. (in: Bacteria) |
90 | - |
pH 5, half-life: 20 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.6 | active in the range | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
retains activity below pH 5 | Bacillus sp. (in: Bacteria) |