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Literature summary for 3.2.1.57 extracted from
- Pullulan 4-glucanohydrolase from Aspergillus niger (1972), Arch. Biochem. Biophys., 153, 180-187.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
gel filtration | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | Aspergillus niger | - |
isopanose + (Glc)4 | small amount | ? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 480 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-glucosylmaltotriose + H2O | - |
Aspergillus niger | isomaltose + maltose | - |
? | |
| alpha-maltosylmaltose + H2O | - |
Aspergillus niger | isopanose + glucose | - |
? | |
| panose + H2O | - |
Aspergillus niger | isomaltose + glucose | - |
? | |
| pullulan + H2O | - |
Aspergillus niger | isopanose + (Glc)4 | small amount | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
at pH 3.5 | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min at pH 3.7-4.5 | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 3.5 | - |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | 5°C, 24 h | Aspergillus niger |
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Release 2023.1 (January 2023)
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