Cloned (Comment) | Organism |
---|---|
Abf62A-Axe6A is a modular enzyme comprising (in order from the N-terminus): an N-terminal signal peptide, a glycoside hydrolase family 62 (GH62) catalytic module, a family 6 carbohydrate binding module (CBM6), a dockerin module and an additional carbohydrate esterase family 6 catalytic module (CE6). Three Abf62A-Axe6A derivatives are constructed, overexpressed in Escherichia coli, purified, and biochemically characterized: 1. RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide, 2. RjAbf62A-CBM6, containing the GH62 and CBM6 modules, and 3. RjAxe6A, containing only CE6 | Ruminiclostridium josui |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84570 | - |
calculated from sequence | Ruminiclostridium josui |
85000 | - |
SDS-PAGE | Ruminiclostridium josui |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminiclostridium josui | - |
- |
- |
Ruminiclostridium josui JCM 1788 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ruminiclostridium josui |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23 | - |
substrate: 2(3),3(3)-di-alpha-L-arabinofuranosyl xylotriose, pH 7.0, 45°C | Ruminiclostridium josui |
410 | - |
substrate: 3(3)-alpha-L-arabinofuranosyl xylotetraose, pH 7.0, 45°C | Ruminiclostridium josui |
1630 | - |
substrate: 2(3)-alpha-L-arabinofuranosyl xylotriose, pH 7.0, 45°C | Ruminiclostridium josui |
2300 | - |
substrate: 3(2)-alpha-L-arabinofuranosyl xylobiose, pH 7.0, 45°C | Ruminiclostridium josui |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2(3),3(3)-di-alpha-L-arabinofuranosyl xylotriose + H2O | low activity | Ruminiclostridium josui | ? | - |
? | |
2(3)-alpha-L-arabinofuranosyl xylotriose + H2O | high activity | Ruminiclostridium josui | arabinose + xylotriose | - |
? | |
3(2)-alpha-L-arabinofuranosyl xylobiose + H2O | high activity | Ruminiclostridium josui | ? | - |
? | |
3(3)-alpha-L-arabinofuranosyl xylotetraose + H2O | high activity | Ruminiclostridium josui | arabinose + xylotetraose | - |
? | |
additional information | hydrolysis of either 1,2- or 1,3-linkage of doubly arabinosylated xylosyl residues in addition to 1,2- and 1,3-linkages of singly arabinosylated xylosyl residues | Ruminiclostridium josui | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000, SDS-PAGE | Ruminiclostridium josui |
Synonyms | Comment | Organism |
---|---|---|
Abf62A-Axe6A | - |
Ruminiclostridium josui |
alpha-L-arabinofuranosidase | - |
Ruminiclostridium josui |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
substrate: alpha-D-cellobiose octaacetate. Enzyme derivative RjAbf62A-CBM6, containing the glycoside hydrolase family 62 (GH62) catalytic module and the family 6 carbohydrate binding module (CBM6) | Ruminiclostridium josui |
55 | - |
RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide | Ruminiclostridium josui |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide | Ruminiclostridium josui |
6 | - |
substrate: alpha-D-cellobiose octaacetate. Enzyme derivative RjAbf62A-CBM6, containing the glycoside hydrolase family 62 (GH62) catalytic module and the family 6 carbohydrate binding module (CBM6) | Ruminiclostridium josui |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 4°C, RjAbf62A-Axe6A, containing all four modules but lacking the signal peptide is stable for 16 h | Ruminiclostridium josui |
6 | 10 | 4°C, enzyme derivative RjAbf62A-CBM6, containing the glycoside hydrolase family 62 (GH62) catalytic module and the family 6 carbohydrate binding module (CBM6)retains its initial activity after a 16 h incubation | Ruminiclostridium josui |