Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Streptomyces sp. SWU10 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 15% residual activity at 1 mM | Streptomyces sp. SWU10 | |
Cu2+ | 18% residual activity at 1 mM | Streptomyces sp. SWU10 | |
EDTA | 30% residual activity at 1 mM | Streptomyces sp. SWU10 | |
Fe2+ | 22% residual activity at 1 mM | Streptomyces sp. SWU10 | |
Fe3+ | 70% residual activity at 1 mM | Streptomyces sp. SWU10 | |
Hg2+ | 59% residual activity at 1 mM | Streptomyces sp. SWU10 | |
Zn2+ | 72% residual activity at 1 mM | Streptomyces sp. SWU10 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 128% activity at 1 mM | Streptomyces sp. SWU10 | |
Ca2+ | 125% activity at 1 mM | Streptomyces sp. SWU10 | |
Mn2+ | 115% activity at 1 mM | Streptomyces sp. SWU10 | |
additional information | not influenced by Na+, Ni2+, K+, and Co2+ | Streptomyces sp. SWU10 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. SWU10 | A0A2Z5VL17 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography and Superdex 75 gel filtration | Streptomyces sp. SWU10 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
cell-free extract, at pH 5.0 and 50°C | Streptomyces sp. SWU10 |
1.29 | - |
after 32.3fold purification, at pH 5.0 and 50°C | Streptomyces sp. SWU10 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birch wood xylan + H2O | - |
Streptomyces sp. SWU10 | alpha-L-arabinofuranose + ? | - |
? | |
additional information | no activity with 4-nitrophenyl alpha-L-arabinofuranoside and 4-nitropheny beta-D-xylopyranoside | Streptomyces sp. SWU10 | ? | - |
? | |
sugar beet arabinan + H2O | - |
Streptomyces sp. SWU10 | alpha-L-arabinofuranose + ? | - |
? | |
sugar beet debranched arabinan + H2O | - |
Streptomyces sp. SWU10 | alpha-L-arabinofuranose + ? | - |
? | |
wheat arabinoxylan + H2O | - |
Streptomyces sp. SWU10 | alpha-L-arabinofuranose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Streptomyces sp. SWU10 |
? | x * 46232, calculated from amino acid sequence | Streptomyces sp. SWU10 |
Synonyms | Comment | Organism |
---|---|---|
Abf62A | - |
Streptomyces sp. SWU10 |
arabinoxylan arabinofuranohydrolase | - |
Streptomyces sp. SWU10 |
AXH | - |
Streptomyces sp. SWU10 |
swu62A | - |
Streptomyces sp. SWU10 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Streptomyces sp. SWU10 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | about 55% activity at 30°C, about 70% activity at 40°C, 100% activity at 50°C, about 45% activity at 60°C | Streptomyces sp. SWU10 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | the enzyme loses all activity after 1 h at 60°C. 60% activity remain after 1 h at 20-50°C | Streptomyces sp. SWU10 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Streptomyces sp. SWU10 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | more than 60% activity between pH 4.0 and 7.0 | Streptomyces sp. SWU10 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.1 | 10.5 | more than 70% of the initial enzymatic activity remain after 1 h at pH 4.1-10.0 | Streptomyces sp. SWU10 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces sp. SWU10 | calculated from amino acid sequence | - |
6.1 |