Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(lambdaDE3) cells | Streptomyces thermoviolaceus |
expressed in Escherichia coli BL21(lambdaDE3) cells | Bifidobacterium adolescentis |
Protein Variants | Comment | Organism |
---|---|---|
R239A | the activity of the mutant enzyme with wheat arabinoxylan is reduced by 100times compared to the wild type enzyme | Streptomyces thermoviolaceus |
R239A/F212A | the activity of the mutant enzyme with wheat arabinoxylan is reduced by 200times compared to the wild type enzyme | Streptomyces thermoviolaceus |
R239A/F212A/I233A | the activity of the mutant enzyme with wheat arabinoxylan is reduced by 8000times compared to the wild type enzyme | Streptomyces thermoviolaceus |
R239A/I233A | the activity of the mutant enzyme with wheat arabinoxylan is reduced by 250times compared to the wild type enzyme | Streptomyces thermoviolaceus |
R239K | the activity of the mutant enzyme with wheat arabinoxylan is reduced by 100times compared to the wild type enzyme | Streptomyces thermoviolaceus |
R239S | the activity of the mutant enzyme with wheat arabinoxylan is reduced by 150times compared to the wild type enzyme | Streptomyces thermoviolaceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | Q5JB56 | - |
- |
Bifidobacterium adolescentis ATCC 15703 | Q5JB56 | - |
- |
Streptomyces thermoviolaceus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Uno-Q column chromatography | Streptomyces thermoviolaceus |
Ni-NTA resin column chromatography and Uno-Q column chromatography | Bifidobacterium adolescentis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
di-2,3-arabinoxylan + H2O | - |
Bifidobacterium adolescentis | ? | - |
? | |
di-2,3-arabinoxylan + H2O | - |
Bifidobacterium adolescentis ATCC 15703 | ? | - |
? | |
mono-2,3-arabinoxylan + H2O | - |
Streptomyces thermoviolaceus | ? | - |
? | |
additional information | no activity with di-2,3-arabinoxylan | Streptomyces thermoviolaceus | ? | - |
? | |
additional information | no activity with mono-2,3-arabinoxylan | Bifidobacterium adolescentis | ? | - |
? | |
additional information | no activity with mono-2,3-arabinoxylan | Bifidobacterium adolescentis ATCC 15703 | ? | - |
? | |
wheat arabinoxylan + H2O | - |
Streptomyces thermoviolaceus | alpha-L-arabinofuranose + ? | - |
? | |
wheat arabinoxylan + H2O | - |
Bifidobacterium adolescentis | alpha-L-arabinofuranose + ? | - |
? | |
wheat arabinoxylan + H2O | - |
Bifidobacterium adolescentis ATCC 15703 | alpha-L-arabinofuranose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35900, calculated from amino acid sequence | Streptomyces thermoviolaceus |
? | x * 59300, calculated from amino acid sequence | Bifidobacterium adolescentis |
Synonyms | Comment | Organism |
---|---|---|
Abf62A | - |
Streptomyces thermoviolaceus |
Abf62A | - |
Bifidobacterium adolescentis |
alpha-L-arabinofuranosidase | - |
Streptomyces thermoviolaceus |
alpha-L-arabinofuranosidase | - |
Bifidobacterium adolescentis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
the enzyme retains 0.03% activity after 1 h of incubation at 55°C | Bifidobacterium adolescentis |
55 | - |
the enzyme retains 73% activity after 1 h of incubation at 55°C | Streptomyces thermoviolaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bifidobacterium adolescentis |
7 | - |
- |
Streptomyces thermoviolaceus |