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Literature summary for 3.2.1.55 extracted from

  • Geng, A.; Wu, J.; Xie, R.; Wang, H.; Wu, Y.; Li, X.; Chang, F.; Sun, J.
    Highly thermostable GH51 alpha-arabinofuranosidase from Hungateiclostridium clariflavum DSM 19732 (2019), Appl. Microbiol. Biotechnol., 103, 3783-3793 .
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acetivibrio clariflavus

Inhibitors

Inhibitors Comment Organism Structure
alpha-L-arabinose the enzyme retains 80.6% activity in the presence of 50 mM and 55.3% activity in the presence of 167 mM Acetivibrio clariflavus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.08
-
 4-nitrophenyl alpha-L-arabinopyranoside at pH 6.5 and 60°C Acetivibrio clariflavus
8.01
-
 2-alpha-L-arabinofuranosyl xylotriose at pH 6.5 and 60°C Acetivibrio clariflavus
32.6
-
 3-alpha-L-arabinofuranosyl xylobiose at pH 6.5 and 60°C Acetivibrio clariflavus
34.4
-
 2,3-di-alpha-L-arabinofuranosyl xylotriose at pH 6.5 and 60°C Acetivibrio clariflavus

Organism

Organism UniProt Comment Textmining
Acetivibrio clariflavus
-
-
-
Acetivibrio clariflavus DSM 19732
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel column chromatography Acetivibrio clariflavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O
-
 Acetivibrio clariflavus alpha-L-arabinose + ?
-
 ?
2,3-di-alpha-L-arabinofuranosyl xylotriose + H2O
-
 Acetivibrio clariflavus DSM 19732 alpha-L-arabinose + ?
-
 ?
2-alpha-L-arabinofuranosyl xylotriose + H2O
-
 Acetivibrio clariflavus alpha-L-arabinose + ?
-
 ?
2-alpha-L-arabinofuranosyl xylotriose + H2O
-
 Acetivibrio clariflavus DSM 19732 alpha-L-arabinose + ?
-
 ?
3-alpha-L-arabinofuranosyl xylobiose + H2O
-
 Acetivibrio clariflavus alpha-L-arabinose + ?
-
 ?
3-alpha-L-arabinofuranosyl xylobiose + H2O
-
 Acetivibrio clariflavus DSM 19732 alpha-L-arabinose + ?
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Acetivibrio clariflavus 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Acetivibrio clariflavus DSM 19732 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
additional information no activity with 4-nitrophenyl beta-D-xylopyranoside Acetivibrio clariflavus ?
-
 ?
additional information no activity with 4-nitrophenyl beta-D-xylopyranoside Acetivibrio clariflavus DSM 19732 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Acetivibrio clariflavus
? x * 56000, calculated from amino acid sequence Acetivibrio clariflavus

Synonyms

Synonyms Comment Organism
AbF51
-
 Acetivibrio clariflavus
GH51 alpha-arabinofuranosidase
-
 Acetivibrio clariflavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Acetivibrio clariflavus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 70 more than 60% activity between 45 and 70°C Acetivibrio clariflavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 at 60°C, the enzyme retains over 90% activity after a 2-day incubation and over 60% activity after a 6-day incubation. The enzyme remains approximately 58% activity after a 1-day incubation at 70°C Acetivibrio clariflavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.9
-
 2,3-di-alpha-L-arabinofuranosyl xylotriose at pH 6.5 and 60°C Acetivibrio clariflavus
55.2
-
 2-alpha-L-arabinofuranosyl xylotriose at pH 6.5 and 60°C Acetivibrio clariflavus
631.4
-
 4-nitrophenyl alpha-L-arabinopyranoside at pH 6.5 and 60°C Acetivibrio clariflavus
743.9
-
 3-alpha-L-arabinofuranosyl xylobiose at pH 6.5 and 60°C Acetivibrio clariflavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Acetivibrio clariflavus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
200
-
 at pH 6.5 and 60°C Acetivibrio clariflavus alpha-L-arabinose

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.5
-
 2,3-di-alpha-L-arabinofuranosyl xylotriose at pH 6.5 and 60°C Acetivibrio clariflavus
6.9
-
 2-alpha-L-arabinofuranosyl xylotriose at pH 6.5 and 60°C Acetivibrio clariflavus
22.8
-
 3-alpha-L-arabinofuranosyl xylobiose at pH 6.5 and 60°C Acetivibrio clariflavus
205
-
 4-nitrophenyl alpha-L-arabinopyranoside at pH 6.5 and 60°C Acetivibrio clariflavus