Literature summary for 3.2.1.55 extracted from

Goyal, A.; Ahmed, S.; Sharma, K.; Gupta, V.; Bule, P.; Alves, V.D.; Fontes, C.M.; Najmudin, S.
Molecular determinants of substrate specificity revealed by the structure of Clostridium thermocellum arabinofuranosidase 43A from glycosyl hydrolase family 43 subfamily 16 (2016), Acta crystallogr. Sect. D, 72, 1281-1289 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) pLysS cells	Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, using either 0.1 M sodium acetate pH 4.5, 2 M ammonium sulfate or 0.1 M Tris pH 8.5, 20% (w/v) PEG 2000 monomethyl ether, 0.2 M trimethylamine N-oxide	Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	A3DEX4	-	-
Acetivibrio thermocellus DSM 1237	A3DEX4	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal affinity column chromatography, and Superdex 75 gel filtration	Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O	-	Acetivibrio thermocellus	4-nitrophenol + alpha-L-arabinofuranose	-	?
4-nitrophenyl alpha-L-arabinofuranoside + H2O	-	Acetivibrio thermocellus DSM 1237	4-nitrophenol + alpha-L-arabinofuranose	-	?

Subunits

Subunits	Comment	Organism
?	x * 36000, SDS-PAGE	Acetivibrio thermocellus
?	x * 35860, calculated from amino acid sequence	Acetivibrio thermocellus

Synonyms

Synonyms	Comment	Organism
Abf43A	-	Acetivibrio thermocellus
arabinofuranosidase 43A	-	Acetivibrio thermocellus

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Release 2023.1 (January 2023)