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Literature summary for 3.2.1.55 extracted from
- Heterologous expression and characterization of alpha-L-arabinofuranosidase 4 from Penicillium purpurogenum and comparison with the other isoenzymes produced by the fungus (2015), Fungal Biol., 119, 641-647.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | enzyme shows synergistic effect on arabinose liberation from wheat arabinoxylan when combined with endoxylanase from Penicillium purpurogenum | Talaromyces purpureogenus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Talaromyces purpureogenus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.58 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 4.6, 37°C | Talaromyces purpureogenus |  |
| 5.3 | - |
4-nitrophenyl beta-D-galactofuranoside | pH 4.6, 37°C | Talaromyces purpureogenus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55400 | - |
- |
Talaromyces purpureogenus |
| 68000 | - |
- |
Talaromyces purpureogenus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces purpureogenus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Talaromyces purpureogenus | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Talaromyces purpureogenus | 4-nitrophenol + beta-D-galactofuranose | - |
? | |
| alpha-1,5-L-arabinobiose + H2O | - |
Talaromyces purpureogenus | 2 L-arabinose | - |
? | |
| alpha-1,5-L-arabinohexaose + 5 H2O | - |
Talaromyces purpureogenus | 6 L-arabinose | - |
? | |
| additional information | no substrates: short arabinooligosaccharides or debranched arabinan | Talaromyces purpureogenus | ? | - |
? | |
| sugar beet arabinan + H2O | - |
Talaromyces purpureogenus | L-arabinofuranose + ? | release of 24.5% of L-arabinofuranose | ? | |
| wheat flour arabinoxylan + H2O | - |
Talaromyces purpureogenus | L-arabinofuranose + ? | release of 23% of L-arabinofuranose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55400, calculated for mature protein, x * 68000, SDS-PAGE | Talaromyces purpureogenus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Abf4 | - |
Talaromyces purpureogenus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Talaromyces purpureogenus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
4-nitrophenyl beta-D-galactofuranoside | pH 4.6, 37°C | Talaromyces purpureogenus | |
| 2.1 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 4.6, 37°C | Talaromyces purpureogenus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Talaromyces purpureogenus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
sharp decrease in activity above | Talaromyces purpureogenus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
4-nitrophenyl beta-D-galactofuranoside | pH 4.6, 37°C | Talaromyces purpureogenus | |
| 1.32 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 4.6, 37°C | Talaromyces purpureogenus | |
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