Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.55 extracted from

  • Valls, A.; Diaz, P.; Pastor, F.I.; Valenzuela, S.V.
    A newly discovered arabinoxylan-specific arabinofuranohydrolase. Synergistic action with xylanases from different glycosyl hydrolase families (2016), Appl. Microbiol. Biotechnol., 100, 1743-1751.
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
individual expression of the GH43 and CBM6 modules in Escherichia coli Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
additional information individually expressed catalytic GH43 module does not show hydrolytic activity, the purified CBM6 binds to soluble arabinoxylan Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 rye arabinoxylan Km value 10.6 mg/ml, pH 6.5, 45°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
-
 Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) A0A0F6VYJ7
-
-
Bacillus sp. (in: Bacteria) BP-7 A0A0F6VYJ7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.7
-
 pH 6.5, 45°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rye arabinoxylan + H2O preferred substrate Bacillus sp. (in: Bacteria) L-arabinose + ?
-
 ?
rye arabinoxylan + H2O preferred substrate Bacillus sp. (in: Bacteria) BP-7 L-arabinose + ?
-
 ?
wheat arabinoxylan + H2O
-
 Bacillus sp. (in: Bacteria) L-arabinose + ?
-
 ?
wheat arabinoxylan + H2O
-
 Bacillus sp. (in: Bacteria) BP-7 L-arabinose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51384, calculated, x * 55000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
Axh43A
-
 Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) calculated
-
 7.4

General Information

General Information Comment Organism
physiological function the enzyme shows synergism with xylanase Xyn11E, leading to a 2.54fold increase in the amount of sugars released. It does not show synergism with the xylanases of families GH10 or GH30 Bacillus sp. (in: Bacteria)