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Literature summary for 3.2.1.55 extracted from
- High-resolution crystal structure of a polyextreme GH43 glycosidase from Halothermothrix orenii with alpha-L-arabinofuranosidase activity (2015), Acta Crystallogr. Sect. F, 71, 338-345.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Halothermothrix orenii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure at 1.10 A resolution. As for other GH43 members, the enzyme folds as a five-bladed -propeller. The structure features a metal-binding site on the propeller axis, near the active site | Halothermothrix orenii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stabilization | Halothermothrix orenii |  |
| Mg2+ | stabilization | Halothermothrix orenii | |
| Mn2+ | pH 7.0, adding 2 mM Mn2+ increases the melting temperature of the enzyme from 63°C to 68.5°C | Halothermothrix orenii | |
| additional information | divalent cation is required | Halothermothrix orenii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halothermothrix orenii | B8CZV1 | - |
- |
| Halothermothrix orenii DSM 9562 | B8CZV1 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.017 | - |
presence of 4 M NaCl and Mg2+, pH 6.5, 50°C | Halothermothrix orenii |
| 0.036 | - |
presence of 4 M NaCl and Mn2+, pH 6.5, 50°C | Halothermothrix orenii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Halothermothrix orenii | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Halothermothrix orenii DSM 9562 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AraF43 | - |
Halothermothrix orenii |
| Hore_20580 | - |
Halothermothrix orenii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 63 | - |
melting temperature | Halothermothrix orenii |
| 68.5 | - |
melting temperatue, presence of 2 mM Mn2+ | Halothermothrix orenii |
| 76 | - |
melting temperature, presence of 4 M NaCl and Mn2+ at pH 6.5 | Halothermothrix orenii |
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Release 2023.1 (January 2023)
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