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Literature summary for 3.2.1.55 extracted from

  • Ahmed, S.; Luis, A.; Bras, J.; Fontes, C.; Goyal, A.
    The family 6 carbohydrate-binding module (CtCBM6B) of Clostridium thermocellum alpha-L-arabinofuranosidase binds xylans and thermally stabilized by Ca2+ ions (2013), Biocatal. Biotransform., 31, 217-225.
No PubMed abstract available
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information substrate binding kinetics of recombinant enzyme Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ increases the thermal stability of the enzyme Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14740
-
 x * 15000, recombinant His-tagged enzyme, SDS-PAGE, x * 14740, about, sequence calculation Acetivibrio thermocellus
15000
-
 x * 15000, recombinant His-tagged enzyme, SDS-PAGE, x * 14740, about, sequence calculation Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
insoluble wheat L-arabinoxylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
oat spelt L-arabinoxylan + H2O higher activity Acetivibrio thermocellus ?
-
 ?
rye L-arabinoxylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 15000, recombinant His-tagged enzyme, SDS-PAGE, x * 14740, about, sequence calculation Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
 Acetivibrio thermocellus
Ct43Araf
-
 Acetivibrio thermocellus
CtCBM6B
-
 Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53 59 Ca2+ increases the melting temperature of the enzyme from 53°C to 59°C Acetivibrio thermocellus

General Information

General Information Comment Organism
evolution the enzyme belongs to glycosyl hydrolase family 43, GH43 Acetivibrio thermocellus