Cloned (Comment) | Organism |
---|---|
gene afq1, overexpression of the enzyme containing thioredoxin, a histidine tag and a S-tag, attached to the N-terminus in Escherichia coli strain BL21(DE3) | Penicillium chrysogenum |
gene afs1, overexpression of the enzyme containing thioredoxin, a His-tag and a S-tag, attached to the N-terminus in Escherichia coli strain BL21(DE3) | Penicillium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49576 | - |
x * 71000, recombinant tagged enzyme, SDS-PAGE, x * 52000 untagged enzyme, SDS-PAGE, x * 49576, enzyme residues 27-506, sequence calculation | Penicillium chrysogenum |
52000 | - |
x * 71000, recombinant tagged enzyme, SDS-PAGE, x * 52000 untagged enzyme, SDS-PAGE, x * 49576, enzyme residues 27-506, sequence calculation | Penicillium chrysogenum |
67496 | - |
x * 85000, recombinant tagged enzyme, SDS-PAGE, x * 79000, untagged enzyme, SDS-PAGE, x * 67496, enzyme residues 18-635, sequence calculation | Penicillium chrysogenum |
71000 | - |
x * 71000, recombinant tagged enzyme, SDS-PAGE, x * 52000 untagged enzyme, SDS-PAGE, x * 49576, enzyme residues 27-506, sequence calculation | Penicillium chrysogenum |
79000 | - |
x * 85000, recombinant tagged enzyme, SDS-PAGE, x * 79000, untagged enzyme, SDS-PAGE, x * 67496, enzyme residues 18-635, sequence calculation | Penicillium chrysogenum |
85000 | - |
x * 85000, recombinant tagged enzyme, SDS-PAGE, x * 79000, untagged enzyme, SDS-PAGE, x * 67496, enzyme residues 18-635, sequence calculation | Penicillium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | B5MGR0 | constitutive gene afq1 | - |
Penicillium chrysogenum | B5MGR1 | inducible gene afs1 | - |
Penicillium chrysogenum 31B | B5MGR0 | constitutive gene afq1 | - |
Penicillium chrysogenum 31B | B5MGR1 | inducible gene afs1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 7 asparagine-linked oligosaccharides for enzyme AFQ1 | Penicillium chrysogenum |
glycoprotein | one asparagine-linked oligosaccharide for enzyme AFS1 | Penicillium chrysogenum |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum | 4-nitrophenol + L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum 31B | 4-nitrophenol + L-arabinofuranose | - |
? | |
debranched arabinan + H2O | - |
Penicillium chrysogenum | ? | - |
? | |
debranched arabinan + H2O | - |
Penicillium chrysogenum 31B | ? | - |
? | |
additional information | substrate specificity, overview. The recombinant enzyme degrades arabinofuranosyl side chains that are both singly and doubly linked to the backbones of arabinoxylan and L-arabinan, NMR spectroscopic analysis | Penicillium chrysogenum | ? | - |
? | |
additional information | substrate specificity, overview. The recombinant enzyme preferentially releases arabinose linked to C-3 of single-substituted xylose or arabinose residues in the two polysaccharides, NMR spectroscopic analysis | Penicillium chrysogenum | ? | - |
? | |
additional information | substrate specificity, overview. The recombinant enzyme preferentially releases arabinose linked to C-3 of single-substituted xylose or arabinose residues in the two polysaccharides, NMR spectroscopic analysis | Penicillium chrysogenum 31B | ? | - |
? | |
additional information | substrate specificity, overview. The recombinant enzyme degrades arabinofuranosyl side chains that are both singly and doubly linked to the backbones of arabinoxylan and L-arabinan, NMR spectroscopic analysis | Penicillium chrysogenum 31B | ? | - |
? | |
sugar beet L-arabinan + H2O | - |
Penicillium chrysogenum | ? | - |
? | |
sugar beet L-arabinan + H2O | - |
Penicillium chrysogenum 31B | ? | - |
? | |
wheat arabinoxylan + H2O | - |
Penicillium chrysogenum | ? | - |
? | |
wheat arabinoxylan + H2O | - |
Penicillium chrysogenum 31B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71000, recombinant tagged enzyme, SDS-PAGE, x * 52000 untagged enzyme, SDS-PAGE, x * 49576, enzyme residues 27-506, sequence calculation | Penicillium chrysogenum |
? | x * 85000, recombinant tagged enzyme, SDS-PAGE, x * 79000, untagged enzyme, SDS-PAGE, x * 67496, enzyme residues 18-635, sequence calculation | Penicillium chrysogenum |
Synonyms | Comment | Organism |
---|---|---|
AFQ1 | - |
Penicillium chrysogenum |
AFS1 | - |
Penicillium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Penicillium chrysogenum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, pH 5.0, stable up to | Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Penicillium chrysogenum |
Organism | Comment | Expression |
---|---|---|
Penicillium chrysogenum | gene afq1 is constitutive | additional information |
Penicillium chrysogenum | gene afs1 is strongly induced by arabinose, arabinitol, arabinan, and arabinoxylan, and by galactan to a lesser extent | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the family 51 glycoside hydrolase, GH51 | Penicillium chrysogenum |
evolution | the enzyme belongs to the family 54 glycoside hydrolase, GH54 | Penicillium chrysogenum |