Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | uncultured rumen bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside | - |
uncultured rumen bacterium | |
4-nitrophenyl beta-D-xylopyranoside | - |
uncultured rumen bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.23 | - |
4-nitrophenyl alpha-L-arabinofuranoside | in 50 mM phosphate/citrate buffer pH 7.0, at 40°C | uncultured rumen bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | uncultured rumen bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured rumen bacterium | E3TBJ3 | from rumen of Bos grunniens | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | uncultured rumen bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
uncultured rumen bacterium | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
additional information | no activity on 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, rice straw xylan or birchwood xylan | uncultured rumen bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | uncultured rumen bacterium |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase | - |
uncultured rumen bacterium |
beta-D-xylosidase/alpha-L-arabinofuranosidase | - |
uncultured rumen bacterium |
RuXyn1 | - |
uncultured rumen bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | the enzyme is relatively stable at 40°C, but loses 76% of its activity after 10 min at 45°C, and 97% of activity at 50°C. The irreversible thermal denaturation half-time of the enzyme is calculated to be 112 min at 40°C, and 5.8 min at 45°C | uncultured rumen bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
4-nitrophenyl alpha-L-arabinofuranoside | in 50 mM phosphate/citrate buffer pH 7.0, at 40°C | uncultured rumen bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
uncultured rumen bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | the enzyme retains over 72% of optimal activity at pH 6.0, at least 40% at pH 5.0, and more than 10% at pH 4.0, however enzyme activity decreases dramatically at pH 8.0, and only 20% of its activity remains | uncultured rumen bacterium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
20.8 | - |
4-nitrophenyl alpha-L-arabinofuranoside | in 50 mM phosphate/citrate buffer pH 7.0, at 40°C | uncultured rumen bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.7 | - |
4-nitrophenyl alpha-L-arabinofuranoside | in 50 mM phosphate/citrate buffer pH 7.0, at 40°C | uncultured rumen bacterium |