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Literature summary for 3.2.1.55 extracted from

  • Ravanal, M.C.; Callegari, E.; Eyzaguirre, J.
    Novel bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (ABF3) from Penicillium purpurogenum (2010), Appl. Environ. Microbiol., 76, 5247-5253.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.55

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.65
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47305
-
 1 * 47305, mature protein, calculated from amino acid sequence Talaromyces purpureogenus
50700
-
 1 * 50700, deduced from SDS-PAGE Talaromyces purpureogenus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
debranched sugar beet arabinan + H2O Talaromyces purpureogenus
-
 L-arabinose + ? 1.35% L-arabinose liberated after 72 h incubation at 28°C ?
sugar beet arabinan + H2O Talaromyces purpureogenus
-
 arabinose + ? 0.82% L-arabinose liberated after 72 h incubation at 28°C ?
wheat flour arabinoxylan + H2O Talaromyces purpureogenus
-
 L-arabinose + ? 15.9% L-arabinose liberated after 72 h incubation at 28°C ?

Organism

Organism UniProt Comment Textmining
Talaromyces purpureogenus C3W4Y2 soft rot fungus
-
Talaromyces purpureogenus ATCC MYA-38 C3W4Y2 soft rot fungus
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Talaromyces purpureogenus

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, SP Sepharose column chromatography and BioGel P-300 gel filtration Talaromyces purpureogenus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.76
-
 crude extract, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus
89.5
-
 purified enzyme, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + L-arabinofuranose
-
 ?
debranched sugar beet arabinan + H2O
-
 Talaromyces purpureogenus L-arabinose + ? 1.35% L-arabinose liberated after 72 h incubation at 28°C ?
additional information the enzyme does not hydrolyze arabinooligosaccharides, 4-nitrophenyl alpha-L-arabinopyranoside, o-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galactopyranoside (less than 2% activity with each substrate) Talaromyces purpureogenus ?
-
 ?
additional information the enzyme does not hydrolyze arabinooligosaccharides, 4-nitrophenyl alpha-L-arabinopyranoside, o-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galactopyranoside (less than 2% activity with each substrate) Talaromyces purpureogenus ATCC MYA-38 ?
-
 ?
sugar beet arabinan + H2O
-
 Talaromyces purpureogenus arabinose + ? 0.82% L-arabinose liberated after 72 h incubation at 28°C ?
wheat flour arabinoxylan + H2O
-
 Talaromyces purpureogenus L-arabinose + ? 15.9% L-arabinose liberated after 72 h incubation at 28°C ?

Subunits

Subunits Comment Organism
monomer 1 * 47305, mature protein, calculated from amino acid sequence Talaromyces purpureogenus
monomer 1 * 50700, deduced from SDS-PAGE Talaromyces purpureogenus

Synonyms

Synonyms Comment Organism
ABF3 bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities Talaromyces purpureogenus
alpha-L-arabinofuranoside arabinofuranohydrolase
-
 Talaromyces purpureogenus
arabinofuranosidase 3 bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities Talaromyces purpureogenus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Talaromyces purpureogenus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 50 the enzyme loses 40% of its activity at 35°C and 90% at 50°C after 30 min of incubation Talaromyces purpureogenus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.73
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Talaromyces purpureogenus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.17
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus