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Literature summary for 3.2.1.55 extracted from
- Characterization of a modular enzyme of exo-1,5-alpha-L-arabinofuranosidase and arabinan binding module from Streptomyces avermitilis NBRC14893 (2008), Appl. Microbiol. Biotechnol., 80, 399-408.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Streptomyces avermitilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE, CBM42-deleted mutant, predicted molecular mass 33000 Da | Streptomyces avermitilis |
| 53000 | - |
SDS-PAGE, recombinant SaAraf43A, predicted molecular mass 52000 Da | Streptomyces avermitilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces avermitilis | Q82P90 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-nitrilotriacetic acid (NTA) agarose column chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) | Streptomyces avermitilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.92 | - |
p-nitrophenyl alpha-L-arabinofuranoside, alpha-L-arabinofuranoside | Streptomyces avermitilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces avermitilis | alpha-L-arabinofuranose | - |
? |  |
| debrached 1,5-arabinan + H2O | - |
Streptomyces avermitilis | ? | - |
? | |
| additional information | does not hydrolyse arabinoxylan, arabinogalactan, gum arabic, and arabinan | Streptomyces avermitilis | ? | - |
? | |
| additional information | does not hydrolyse methyl 2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside, methyl 3-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | Streptomyces avermitilis | ? | - |
? | |
| additional information | does not hydrolyze p-nitrophenyl-alpha-L-arabinopyranoside, p-nitrophenyl-beta-D-xylopyranoside, or p-nitrophenyl-beta-D-galactopyranoside | Streptomyces avermitilis | ? | - |
? | |
| p-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces avermitilis | p-nitrophenol + L-arabinofuranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Streptomyces avermitilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Streptomyces avermitilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
more than 80% activity is retained after incubation at 30°C for 60 min | Streptomyces avermitilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Streptomyces avermitilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | - |
Streptomyces avermitilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.5 | 30°C, more than 80% of the activity is retained | Streptomyces avermitilis |
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