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Literature summary for 3.2.1.55 extracted from

  • Minic, Z.; Do, C.T.; Rihouey, C.; Morin, H.; Lerouge, P.; Jouanin, L.
    Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis (2006), J. Exp. Bot., 57, 2339-2351.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
p-nitrophenyl-alpha-L-arabinofuranoside 37°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arabinan + H2O Arabidopsis thaliana XYL3 XYL3 is able to degrade all terminal arabinosyl residues and participates in the in-vivo hydrolysis of arabinan L-arabinose + ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seed AtBX3 mRNA is specifically expressed in the early stage of seed formation and not at seed maturation Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinan + H2O XYL3 XYL3 is able to degrade all terminal arabinosyl residues and participates in the in-vivo hydrolysis of arabinan Arabidopsis thaliana L-arabinose + ?
-
?
arabinoxylan + H2O
-
Arabidopsis thaliana L-arabinose + ?
-
?
debranched arabinan + H2O
-
Arabidopsis thaliana L-arabinose + ?
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Arabidopsis thaliana p-nitrophenol + L-arabinofuranose
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O 15% of the activity with p-nitrophenyl-alpha-L-arabinofuranoside Arabidopsis thaliana p-nitrophenol + beta-D-xylopyranose
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O 34% of the activity with p-nitrophenyl-alpha-L-arabinofuranoside Arabidopsis thaliana p-nitrophenol + beta-D-xylopyranose
-
?
sugar beet arabinan + H2O the enzyme hydrolyses both arabinosyl-substituted side group residues and terminal arabinofuranosyl residues (1,5)-alpha-linked to the arabinan backbone Arabidopsis thaliana L-arabinose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
XYL3
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.36
-
p-nitrophenyl-alpha-L-arabinofuranoside 37°C Arabidopsis thaliana