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Literature summary for 3.2.1.55 extracted from
- Synergy between an alpha-L-arabinofuranosidase from Aspergillus oryzae and an endo-arabinanase from Streptomyces coelicolor for degradation of arabinan. (2006), Food Sci. Technol. Res., 12, 43-49.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Aspergillus oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
x * 61000, SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | Q2UIM2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32 | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the recombinant enzyme releases L-arabinose from arabinose-containing polysaccharides such as lupin pectic galactan, corn hull arabinoxylan, sugar beet arabinan, and potato pectic galactan. Synergisms between the alpha-L-arabinofuranosidase and the endo-arabinanase from Streptomyces coelicolor for the degradation of arabinan and debranched arabinan are observed. The hydrolysis is most effcient when alphga-L-arabinofuranosidase and endo-arabinanase are in a ratio of 95:5 | Aspergillus oryzae | ? | - |
? |  |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus oryzae | p-nitrophenol + L-arabinofuranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-Afase | - |
Aspergillus oryzae |
| alpha-L-arabinofuranosidase B | - |
Aspergillus oryzae |
| AoAra54A | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | 30°C, 1 h, the enzyme retains almost full activity at pH values ranging from 3.0 to 6.0 | Aspergillus oryzae |
| 7 | - |
30°C, 1 h, activity is reduced by 80% | Aspergillus oryzae |
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