Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Bacillus pumilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 15% inhibition | Bacillus pumilus | |
Hg2+ | 1 mM, complete inhibition | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
p-nitrophenyl alpha-L-arabinofuranoside | - |
Bacillus pumilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus pumilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM, 1.8fold activation | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | Q2MJP5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus pumilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Bacillus pumilus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme releases arabinose from oat spelt xylan. No activity is detected with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-xylopyranoside, and 4-nitrophenyl beta-D-xylopyranoside | Bacillus pumilus | ? | - |
? | |
p-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus pumilus | p-nitrophenol + L-arabinofuranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AraB | - |
Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus pumilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 75 | 45°C: about 70% of maximal activity, 75°C: about 65% of maximal activity, substrate: p-nitrophenyl alpha-L-arabinofuranoside | Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-ife: 1 h | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
substrate: p-nitrophenyl alpha-L-arabinofuranoside | Bacillus pumilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 7.6 | pH 5.2: about 60% of maximal activity, pH 7.6: about 60% of maximal activity, substrate: p-nitrophenyl alpha-L-arabinofuranoside | Bacillus pumilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | 7.6 | 65°C, 1 h, stable | Bacillus pumilus |