Cloned (Comment) | Organism |
---|---|
xynD is isolated from genomic DNA of Bacillus subtilis subsp. subtilis ATCC 6051 and cloned for cytoplasmatic expression in Escherichia coli | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52200 | - |
MALDI-TOF-MS | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q45071 | subsp. subtilis ATCC 6051 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan polymer + H2O | - |
Bacillus subtilis | alpha-L-arabinose + ? | - |
? | |
arabinoxylo-oligosaccharide + H2O | - |
Bacillus subtilis | alpha-L-arabinose + ? | - |
? | |
additional information | XynD specifically releases arabinofuranosyl groups from mono-substituted C-(O)-2 and C-(O)-3 xylopyranosyl residues on the xylan backbone. XynD does not display endoxylanase, xylosidase or arabinanase activity and is inactive on arabinan | Bacillus subtilis | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinofuranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XynD | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
substrate: p-nitrophenyl-alpha-L-arabinofuranoside | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 60% of maximal activity, 50°C: about 60% of maximal activity | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | 40 min, stable | Bacillus subtilis |
60 | - |
40 min, complete and irreversible inactivation | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
substrate: p-nitrophenyl-alpha-L-arabinofuranoside | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | pH 5.0: about 20% of maximal activity, pH 6.0: about 80% of maximal activity | Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | isoelectric focusing revealed a pI to be at least 9.3 | - |
additional information |