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Literature summary for 3.2.1.55 extracted from
- A novel GH43 alpha-L-arabinofuranosidase from Humicola insolens: mode of action and synergy with GH51 alpha-L-arabinofuranosidases on wheat arabinoxylan (2006), Appl. Microbiol. Biotechnol., 73, 850-861.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Aspergillus oryzae, GH43 alpha-L-arabinofuranosidase | Humicola insolens |
| GH51 alpha-L-arabinofuranosidase | Humicola insolens |
| GH51 alpha-L-arabinofuranosidase | Meripilus giganteus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, GH43 alpha-L-arabinofuranosidase, SDS-PAGE | Humicola insolens |
| 69000 | - |
x * 69000, GH51 alpha-L-arabinofuranosidase, SDS-PAGE | Meripilus giganteus |
| 94000 | - |
x * 94000, GH51 alpha-L-arabinofuranosidase, SDS-PAGE | Humicola insolens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium sp. | - |
- |
- |
| Humicola insolens | - |
- |
- |
| Meripilus giganteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GH43 alpha-L-arabinofuranosidase | Humicola insolens |
| GH51 alpha-L-arabinofuranosidase | Humicola insolens |
| GH51 alpha-L-arabinofuranosidase | Meripilus giganteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | GH51 alpha-L-arabinofuranosidase | Meripilus giganteus | 4-nitrophenol + L-arabinofuranose | - |
? |  |
| arabinoxylan + H2O | GH43 enzyme selectively hydrolyses (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides. Combinations (50:50) of the GH43 alpha-AraF from Bifidobacterium sp. and the GH51 alpha-AraFs from Meripilus giganteus or Humicola insolens results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan | Bifidobacterium sp. | ? | - |
? | |
| arabinoxylan + H2O | GH43 enzyme selectively hydrolyses (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus or Humicola insolens results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C | Humicola insolens | ? | - |
? | |
| arabinoxylan + H2O | GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from single substituted xylopyranosyls in arabinoxylan. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C | Meripilus giganteus | ? | - |
? | |
| arabinoxylan + H2O | GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from singly substituted xylopyranosyls in arabinoxylan. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C | Humicola insolens | ? | - |
? | |
| arabinoxylo-oligosaccharides + H2O | GH43 enzyme selectively hydrolysed (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides | Humicola insolens | ? | - |
? | |
| arabinoxylo-oligosaccharides + H2O | GH43 enzyme selectively hydrolysed (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides | Bifidobacterium sp. | ? | - |
? | |
| arabinoxylo-oligosaccharides + H2O | GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from singly substituted xylopyranosyls in arabinoxylan | Humicola insolens | ? | - |
? | |
| arabinoxylo-oligosaccharides + H2O | GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from singly substituted xylopyranosyls in arabinoxylan | Meripilus giganteus | ? | - |
? | |
| additional information | no activity with p-nitrophenyl-alpha-L-arabinofuranoside, GH43 alpha-L-arabinofuranosidase | Humicola insolens | ? | - |
? | |
| additional information | no activity with p-nitrophenyl-alpha-L-arabinofuranoside, GH43 alpha-L-arabinofuranosidase | Bifidobacterium sp. | ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | GH51 alpha-L-arabinofuranosidase | Humicola insolens | p-nitrophenol + L-arabinofuranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, GH43 alpha-L-arabinofuranosidase, SDS-PAGE | Humicola insolens |
| ? | x * 69000, GH51 alpha-L-arabinofuranosidase, SDS-PAGE | Meripilus giganteus |
| ? | x * 94000, GH51 alpha-L-arabinofuranosidase, SDS-PAGE | Humicola insolens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-AraF | - |
Humicola insolens |
| alpha-AraF | - |
Bifidobacterium sp. |
| alpha-AraF | - |
Meripilus giganteus |
| GH43 alpha-L-arabinofuranosidase | - |
Humicola insolens |
| GH43 alpha-L-arabinofuranosidase | - |
Bifidobacterium sp. |
| GH51 alpha-L-arabinofuranosidase | - |
Humicola insolens |
| GH51 alpha-L-arabinofuranosidase | - |
Meripilus giganteus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH43 alpha-L-arabinofuranosidase. Arabinose release catalysed by the Bifidobacterium sp. GH43 enzyme is almost nil outside the exact optimum | Bifidobacterium sp. |
| 48 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH51 alpha-L-arabinofuranosidase | Meripilus giganteus |
| 53 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH43 alpha-L-arabinofuranosidase | Humicola insolens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH51 alpha-L-arabinofuranosidase | Meripilus giganteus |
| 6 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH43 alpha-L-arabinofuranosidase. Arabinose release catalysed by the Bifidobacterium sp. GH43 enzyme is almost nil outside the exact optimum | Bifidobacterium sp. |
| 6 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH51 alpha-L-arabinofuranosidase | Humicola insolens |
| 6.7 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH43 alpha-L-arabinofuranosidase | Humicola insolens |
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Release 2023.1 (January 2023)
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