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Literature summary for 3.2.1.55 extracted from
- Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum (2006), Biochem. J., 395, 31-37.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 DE3 cells | Acetivibrio thermocellus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor diffusion | Acetivibrio thermocellus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0055 | - |
wheat arabinoxylan | - |
Acetivibrio thermocellus | |
| 0.25 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Acetivibrio thermocellus |  |
| 0.55 | - |
alpha-1,5-arabinotriose | - |
Acetivibrio thermocellus | |
| 0.95 | - |
alpha-1,3-arabinoxylobiose | - |
Acetivibrio thermocellus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-1,3-arabinoxylobiose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha-1,5-arabinotriose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Acetivibrio thermocellus | p-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| wheat arabinoxylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | anion exchange chromatography | Acetivibrio thermocellus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Acetivibrio thermocellus |
| Araf51A | - |
Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 82 | - |
with 1 mM p-nitrophenyl-alpha-L-arabinofuranoside as substrate | Acetivibrio thermocellus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
wheat arabinoxylan | - |
Acetivibrio thermocellus | |
| 4.6 | - |
alpha-1,5-arabinotriose | - |
Acetivibrio thermocellus | |
| 8.6 | - |
alpha-1,3-arabinoxylobiose | - |
Acetivibrio thermocellus | |
| 103 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 7.0, 37°C | Acetivibrio thermocellus | |
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Release 2023.1 (January 2023)
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