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Literature summary for 3.2.1.55 extracted from

  • Panagiotou, G.; Topakas, E.; Economou, L.; Kekos, D.; Macris, B.J.; Christakopoulos, P.
    Induction, purification, and characterization of two extracellular alpha-L-arabinofuranosidases from Fusarium oxysporum (2003), Can. J. Microbiol., 49, 639-644.
    View publication on PubMed
Search for more literature for 3.2.1.55

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 45°C, isozyme ABF2 Fusarium oxysporum
0.39
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 45°C, isozyme ABF1 Fusarium oxysporum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Fusarium oxysporum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 isozyme ABF2 Fusarium oxysporum
200000
-
 isozyme ABF1 Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
 2 isozymes ABF1 and ABF2
-

Purification (Commentary)

Purification (Comment) Organism
ABF1, 7.9fold, and ABF2, 20.4fold, to homogeneity Fusarium oxysporum

Source Tissue

Source Tissue Comment Organism Textmining
additional information effect of carbon sources in the medium on enzyme production of the culture, overview Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.88
-
 purified isozyme ABF1 Fusarium oxysporum
10
-
 purified isozyme ABF2 Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Fusarium oxysporum 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
arabinan + H2O from sugar beet, not from wheat Fusarium oxysporum alpha-L-arabinofuranose
-
 ?
arabinoxylan + H2O soluble or insoluble Fusarium oxysporum alpha-L-arabinofuranose + ?
-
 ?
additional information no activity with substrates containing ferulic acid ester linked to C5 and C2 linkages of 4-nitrophenyl alpha-L-arabinofuranoside Fusarium oxysporum ?
-
 ?

Subunits

Subunits Comment Organism
trimer
-
 Fusarium oxysporum

Synonyms

Synonyms Comment Organism
ABF
-
 Fusarium oxysporum
alpha-L-arabinofuranosidase
-
 Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55 isozyme ABF2 Fusarium oxysporum
55 60 isozyme ABF1 Fusarium oxysporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, complete loss of activity of isozyme ABF1, 37% remaining activity of isozyme ABF2 Fusarium oxysporum

pI Value

Organism Comment pI Value Maximum pI Value
Fusarium oxysporum isozyme ABF1
-
 6
Fusarium oxysporum isozyme ABF2
-
 7.1