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Literature summary for 3.2.1.55 extracted from
- Barley arabinoxylan arabinofuranohydrolases: Purification, characterization and determination of primary structures from cDNA clones (2001), Biochem. J., 356, 181-189.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA clones isolated | Hordeum vulgare |
| cDNA isolation | Streptomyces coelicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.77 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Hordeum vulgare |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
- |
Bacillus subtilis |
| 65000 | - |
SDS-PAGE | Hordeum vulgare |
| 68280 | - |
isoenzyme AXAH, encoded by cDNA | Hordeum vulgare |
| 68760 | - |
isoenzyme AXAH-II, encoded by cDNA | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beet arabinan + H2O | Bacillus subtilis | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Geobacillus stearothermophilus | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Hordeum vulgare | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Streptomyces chartreusis | - |
L-arabinose + ? | - |
ir | |
| larchwood arabinogalactan + H2O | Hordeum vulgare | - |
L-arabinose + ? | - |
? | |
| sugar beet arabinan + H2O | Hordeum vulgare | - |
L-arabinose + ? | - |
? | |
| wheat-flour arabinoxylan + H2O | Hordeum vulgare | - |
L-arabinofuranose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Aspergillus niger | P42254 | - |
- |
| Bacillus sp. (in: Bacteria) | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Bacteroides ovatus | Q59218 | - |
- |
| Breznakibacter xylanolyticus | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Hordeum vulgare | - |
L.cv. Clipper | - |
| Streptomyces chartreusis | P82593 | - |
- |
| Streptomyces coelicolor | - |
- |
- |
| Thermoclostridium stercorarium | O08457 | - |
- |
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
- |
Streptomyces chartreusis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| caryopsis | - |
Hordeum vulgare | - |
| seedling | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,5-alpha-L-arabinofuranohexaose + H2O | - |
Hordeum vulgare | L-arabinose + L-arabinopentaose | - |
? | |
| 1,5-alpha-L-arabinofuranohexaose + H2O | - |
Aspergillus niger | L-arabinose + L-arabinopentaose | - |
? | |
| beet arabinan + H2O | - |
Bacillus subtilis | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Hordeum vulgare | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Streptomyces chartreusis | L-arabinose + ? | - |
ir | |
| L-arabinan + H2O | - |
Bacillus subtilis | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Geobacillus stearothermophilus | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Hordeum vulgare | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Aspergillus niger | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Streptomyces chartreusis | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Hordeum vulgare | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Streptomyces chartreusis | L-arabinose + ? | - |
? | |
| L-arabinoxylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Hordeum vulgare | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Streptomyces chartreusis | L-arabinose + ? | - |
ir | |
| larchwood arabinogalactan + H2O | - |
Hordeum vulgare | L-arabinose + ? | - |
? | |
| additional information | - |
Bacillus subtilis | ? | - |
? | |
| additional information | - |
Geobacillus stearothermophilus | ? | - |
? | |
| additional information | - |
Aspergillus niger | ? | - |
? | |
| additional information | - |
Streptomyces chartreusis | ? | - |
? | |
| additional information | enzyme is unable to hydrolyse 4-nitrophenyl arabinopyranosides, xylopyranosides, mannopyranosides, glucopyranosides or galactopyranosides, enzyme does not hydrolyse laminarin, beta-glucan, gum arabic or carboxymethylxylan | Hordeum vulgare | ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Hordeum vulgare | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces chartreusis | p-nitrophenol + L-arabinose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Hordeum vulgare | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Arabidopsis thaliana | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus sp. (in: Bacteria) | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces coelicolor | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Thermotoga maritima | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Breznakibacter xylanolyticus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Bacteroides ovatus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces chartreusis | L-arabinose + phenol | - |
ir | |
| sugar beet arabinan + H2O | - |
Hordeum vulgare | L-arabinose + ? | - |
? | |
| wheat-flour arabinoxylan + H2O | - |
Hordeum vulgare | L-arabinofuranose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arabinoxylan arabinofuranohydrolase | - |
Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
- |
Hordeum vulgare |
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