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Literature summary for 3.2.1.55 extracted from

  • Manin, C.; Shareek, F.; Morosoli, R.; Klueppel, D.
    Purification and characterization of an alpha-L-arabinofuranosidase from Streptomyces lividans 66 and DNA sequence of the gene (abfA) (1994), Biochem. J., 302, 443-449.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene abfA encoding alpha-L-arabinofuranosidase homologously expressed in Streptomyces lividans by complementation of mutant IAF10-164 and DNA sequence determined, multicopy vector pIJ702 permits overproduction of the enzyme Streptomyces lividans
gene xylB encoding AF, heterologously cloned in Escherichia coli Butyrivibrio fibrisolvens
gene, encoding AF, heterologously cloned in Escherichia coli Aspergillus niger
gene, encoding AF, heterologously expressed in Escherichia coli JM83 Thermoclostridium stercorarium
xynC gene, encoding AF, heterologously expressed in Escherichia coli Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Streptomyces lividans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
6 * 69000, SDS-PAGE Streptomyces lividans
72490
-
calculated from DNA and amino acid sequence analysis Streptomyces lividans
380000
-
gel filtration Streptomyces lividans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Bacillus subtilis
-
L-arabinose + ?
-
ir
beet arabinan + H2O Aspergillus niger
-
L-arabinose + ?
-
ir
beet arabinan + H2O Butyrivibrio fibrisolvens
-
L-arabinose + ?
-
?
beet arabinan + H2O Streptomyces sp.
-
L-arabinose + ?
-
ir
beet arabinan + H2O Clostridium acetobutylicum
-
L-arabinose + ?
-
ir
beet arabinan + H2O Streptomyces lividans
-
L-arabinose + ?
-
ir
beet arabinan + H2O Athelia rolfsii
-
L-arabinose + ?
-
ir
beet arabinan + H2O Streptomyces purpurascens
-
L-arabinose + ?
-
?
rye flour arabinoxylan + H2O Streptomyces lividans
-
L-arabinofuranose + ?
-
?
wheat-flour arabinoxylan + H2O Streptomyces lividans
-
L-arabinofuranose + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Athelia rolfsii
-
-
-
Bacillus subtilis
-
-
-
Butyrivibrio fibrisolvens
-
-
-
Clostridium acetobutylicum
-
-
-
Dichomitus squalens
-
-
-
Pseudomonas fluorescens
-
-
-
Streptomyces lividans
-
mutant IAF10-164
-
Streptomyces lividans
-
66strain 1326
-
Streptomyces lividans 66 1326
-
66strain 1326
-
Streptomyces purpurascens
-
-
-
Streptomyces sp.
-
no. 17-1
-
Streptomyces sp. 17-1
-
no. 17-1
-
Thermoclostridium stercorarium
-
-
-
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger
-
Trichoderma reesei
-
Streptomyces lividans
-
Athelia rolfsii
-
Dichomitus squalens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
153
-
-
Streptomyces lividans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylobiose + H2O
-
Butyrivibrio fibrisolvens L-arabinose + xylobiose
-
?
arabinoxylobiose + H2O
-
Streptomyces lividans L-arabinose + xylobiose
-
?
arabinoxylobiose + H2O
-
Streptomyces lividans 66 1326 L-arabinose + xylobiose
-
?
beet arabinan + H2O
-
Bacillus subtilis L-arabinose + ?
-
ir
beet arabinan + H2O
-
Aspergillus niger L-arabinose + ?
-
ir
beet arabinan + H2O
-
Butyrivibrio fibrisolvens L-arabinose + ?
-
?
beet arabinan + H2O
-
Streptomyces sp. L-arabinose + ?
-
ir
beet arabinan + H2O
-
Clostridium acetobutylicum L-arabinose + ?
-
ir
beet arabinan + H2O
-
Streptomyces lividans L-arabinose + ?
-
ir
beet arabinan + H2O
-
Athelia rolfsii L-arabinose + ?
-
ir
beet arabinan + H2O
-
Streptomyces purpurascens L-arabinose + ?
-
?
L-arabinan + H2O
-
Bacillus subtilis (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Clostridium acetobutylicum (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces lividans (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces purpurascens (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinogalactan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
L-arabinogalactan + H2O
-
Streptomyces sp. L-arabinose + ?
-
?
L-arabinogalactan + H2O
-
Thermoclostridium stercorarium L-arabinose + ?
-
?
L-arabinogalactan + H2O
-
Dichomitus squalens L-arabinose + ?
-
?
L-arabinogalactan + H2O
-
Streptomyces sp. 17-1 L-arabinose + ?
-
?
L-arabinoxylan + H2O
-
Aspergillus niger L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Streptomyces sp. L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Trichoderma reesei L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Streptomyces lividans L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Thermoclostridium stercorarium L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Streptomyces sp. 17-1 L-arabinose + ?
-
ir
L-arabinoxylohexaose + H2O
-
Streptomyces lividans L-arabinose + ?
-
?
L-arabinoxylopentaose + H2O
-
Streptomyces lividans L-arabinose + ?
-
?
L-arabinoxylotetraose + H2O
-
Streptomyces lividans L-arabinose + ?
-
?
L-arabinoxylotetraose + H2O
-
Streptomyces lividans 66 1326 L-arabinose + ?
-
?
L-arabinoxylotriose + H2O
-
Streptomyces lividans L-arabinose + ?
-
?
L-arabinoxylotriose + H2O
-
Streptomyces lividans 66 1326 L-arabinose + ?
-
?
additional information
-
Bacillus subtilis ?
-
?
additional information
-
Aspergillus niger ?
-
?
additional information
-
Butyrivibrio fibrisolvens ?
-
?
additional information
-
Streptomyces sp. ?
-
?
additional information
-
Clostridium acetobutylicum ?
-
?
additional information no activity on p-nitrophenyl glycosides like alpha-L-arabinopyranose, beta-D-glucose, beta-D-galactopyranose, beta-D-cellobiose, alpha-L-mannopyranose, oat spelts xylan, xylan or arabinogalactan Streptomyces lividans ?
-
?
additional information inactive towards arabinan, arabinoxylan and arabinogalactan Streptomyces purpurascens ?
-
?
additional information
-
Streptomyces sp. 17-1 ?
-
?
additional information no activity on p-nitrophenyl glycosides like alpha-L-arabinopyranose, beta-D-glucose, beta-D-galactopyranose, beta-D-cellobiose, alpha-L-mannopyranose, oat spelts xylan, xylan or arabinogalactan Streptomyces lividans 66 1326 ?
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aspergillus niger p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces sp. p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Clostridium acetobutylicum p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma reesei p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces lividans p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Thermoclostridium stercorarium p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces purpurascens p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces sp. 17-1 p-nitrophenol + L-arabinose
-
?
phenyl alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Aspergillus niger L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Pseudomonas fluorescens L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces sp. L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Clostridium acetobutylicum L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Trichoderma reesei L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces lividans L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Thermoclostridium stercorarium L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Athelia rolfsii L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces purpurascens L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Dichomitus squalens L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces sp. 17-1 L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces lividans 66 1326 L-arabinose + phenol
-
ir
rye flour arabinoxylan + H2O
-
Streptomyces lividans L-arabinofuranose + ?
-
?
wheat-flour arabinoxylan + H2O
-
Streptomyces lividans L-arabinofuranose + ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 69000, SDS-PAGE Streptomyces lividans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus subtilis
60
-
-
Aspergillus niger
60
-
-
Trichoderma reesei
60
-
-
Streptomyces lividans
60
-
-
Dichomitus squalens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
68
-
half-life 12 min Streptomyces lividans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Butyrivibrio fibrisolvens
6
-
-
Streptomyces sp.
6
-
-
Streptomyces lividans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
stable for 12 h at 4°C Streptomyces lividans