Any feedback?
Literature summary for 3.2.1.55 extracted from
- Characterization and delignification activity of a thermostable alpha-L-arabinofuranosidase from Bacillus stearothermophilus (1993), Appl. Microbiol. Biotechnol., 40, 57-62.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| paper production | - |
Aspergillus niger |
| paper production | extraction of lignin from wood chips, essential step in the preparation of pulp fibres | Geobacillus stearothermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Geobacillus stearothermophilus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Geobacillus stearothermophilus | - |
- |
| extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52500 | - |
1 * 52500 + 1 * 57500, SDS-PAGE | Geobacillus stearothermophilus |
| 57500 | - |
1 * 52500 + 1 * 57500, SDS-PAGE | Geobacillus stearothermophilus |
| 108000 | - |
gel filtration, Sepharose Cl 6B | Geobacillus stearothermophilus |
| 110000 | - |
gel filtration | Geobacillus stearothermophilus |
| 114800 | - |
gel filtration, Superose 12 | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| softwood Kraft pulp + H2O | Geobacillus stearothermophilus | - |
lignin + ? | - |
? | |
| softwood Kraft pulp + H2O | Geobacillus stearothermophilus L1 | - |
lignin + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Geobacillus stearothermophilus | - |
L1 | - |
| Geobacillus stearothermophilus L1 | - |
L1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 101 | - |
- |
Geobacillus stearothermophilus |
| 126 | - |
purified enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | low activity on the substrate | Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | low activity on the substrate | Geobacillus stearothermophilus L1 | L-arabinose + ? | - |
? | |
| L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | low activity on the substrate | Geobacillus stearothermophilus | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | low activity on the substrate | Geobacillus stearothermophilus L1 | L-arabinose + ? | - |
ir | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus L1 | p-nitrophenol + L-arabinose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus L1 | L-arabinose + phenol | - |
ir | |
| softwood Kraft pulp + H2O | - |
Geobacillus stearothermophilus | lignin + ? | - |
? | |
| softwood Kraft pulp + H2O | - |
Geobacillus stearothermophilus L1 | lignin + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 52500 + 1 * 57500, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
at pH 7 and pH 8 | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | at pH 7 enzyme is completely stable at 60°C for at least 80 min, retains 50% of its maximum activity after 75 min at 70°C and loses all activity after 15 min at 80°C | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Geobacillus stearothermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
