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Literature summary for 3.2.1.55 extracted from

  • Whitaker, J.R.
    Pectic substances, pectic enzymes and haze formation in fruit juices (1984), Enzyme Microb. Technol., 6, 341-349.
No PubMed abstract available
Search for more literature for 3.2.1.55

Application

Application Comment Organism
nutrition food industry, preparation of wines and fruit juices Bacillus subtilis
nutrition food industry, preparation of wines and fruit juices Aspergillus niger
nutrition food industry, preparation of wines and fruit juices Athelia rolfsii

Inhibitors

Inhibitors Comment Organism Structure
Mercuric acetate
-
 Aspergillus niger
Sodium tetrathionate
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 L-arabinan
-
 Aspergillus niger
2
-
 (1-5)-L-arabinan
-
 Aspergillus niger
2.83
-
 phenyl alpha-L-arabinofuranoside
-
 Athelia rolfsii
4.7
-
 phenyl alpha-L-arabinofuranoside
-
 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
-
 Aspergillus niger
65000
-
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Bacillus subtilis
-
 L-arabinose + ?
-
 ir
beet arabinan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ir
beet arabinan + H2O Athelia rolfsii
-
 L-arabinose + ?
-
 ir
beet arabinan + H2O Bacillus subtilis F-11
-
 L-arabinose + ?
-
 ir
gum arabic + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
wheat L-arabino-D-xylan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger 5195
-
-
-
Athelia rolfsii
-
-
-
Bacillus subtilis
-
 F-11
-
Bacillus subtilis F-11
-
 F-11
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis
-
 Aspergillus niger
-
 Athelia rolfsii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-L-arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
1,5-L-arabinan + H2O
-
 Athelia rolfsii L-arabinose + ?
-
 ir
1,5-L-arabinan + H2O
-
 Aspergillus niger 5195 L-arabinose + ?
-
 ir
beet arabinan + H2O
-
 Bacillus subtilis L-arabinose + ?
-
 ir
beet arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
beet arabinan + H2O
-
 Athelia rolfsii L-arabinose + ?
-
 ir
beet arabinan + H2O
-
 Bacillus subtilis F-11 L-arabinose + ?
-
 ir
gum arabic + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
L-arabinan + H2O
-
 Bacillus subtilis (1,5-alpha-L-arabinose)25 + ?
-
 ir
L-arabinan + H2O
-
 Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
 ir
L-arabinan + H2O
-
 Bacillus subtilis F-11 (1,5-alpha-L-arabinose)25 + ?
-
 ir
L-arabinan + H2O
-
 Aspergillus niger 5195 (1,5-alpha-L-arabinose)25 + ?
-
 ir
additional information
-
 Aspergillus niger ?
-
 ?
additional information arabinofuranosidase I does not hydrolyze L-arabinan, arabinofuranosidase II has no activity on polygalacturonic acid, carboxymethylcellulose, larch galactan, citrus pectic galactan or the synthetic substrates p-nitrophenyl derivatives of alpha- and beta-D-xylopyranosides, alpha- and beta-D-glucopyranosides, beta-D-fucopyranoside and beta-D-galactopyranoside Bacillus subtilis ?
-
 ?
additional information arabinofuranosidase I does not hydrolyze L-arabinan, arabinofuranosidase II has no activity on polygalacturonic acid, carboxymethylcellulose, larch galactan, citrus pectic galactan or the synthetic substrates p-nitrophenyl derivatives of alpha- and beta-D-xylopyranosides, alpha- and beta-D-glucopyranosides, beta-D-fucopyranoside and beta-D-galactopyranoside Bacillus subtilis F-11 ?
-
 ?
additional information
-
 Aspergillus niger 5195 ?
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O arabinofuranosidase I Bacillus subtilis p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O arabinofuranosidase I Bacillus subtilis F-11 p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger 5195 p-nitrophenol + L-arabinose
-
 ?
phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Athelia rolfsii L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis F-11 L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger 5195 L-arabinose + phenol
-
 ir
wheat L-arabino-D-xylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 enzyme is stable for 10 min at 50°C from pH 3-pH 8, at 60°C stable at pH 4 for 10 min, less stable at pH 5.5 and stable again at pH 7 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
 Athelia rolfsii
3.8 4
-
 Aspergillus niger
6.5
-
-
 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.5 10 acid stable, even after storage at pH 2 and 5°C for 72 h, 83% enzymic activity remains Athelia rolfsii
7
-
-
 Aspergillus niger