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Literature summary for 3.2.1.55 extracted from

  • Komae, K.; Kaji, A.; Sato, M.
    An alpha-L-arabinofuranosidase from Streptomyces purpurascens IFO 3389 (1982), Agric. Biol. Chem., 46, 1899-1905.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Aspergillus niger
Ag+
-
Rhodotorula flava
Ag+
-
Streptomyces purpurascens
Hg2+
-
Pectobacterium carotovorum
Hg2+
-
Rhodotorula flava
Hg2+
-
Streptomyces purpurascens
L-arabino-gamma-lactone
-
Streptomyces purpurascens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Streptomyces purpurascens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
8 * 62000 Streptomyces purpurascens
495000
-
gel filtration Streptomyces purpurascens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Bacillus subtilis
-
L-arabinose + ?
-
?
beet arabinan + H2O Aspergillus niger
-
L-arabinose + ?
-
?
beet arabinan + H2O Pectobacterium carotovorum
-
L-arabinose + ?
-
?
beet arabinan + H2O Athelia rolfsii
-
L-arabinose + ?
-
?
beet arabinan + H2O Rhodotorula flava
-
L-arabinose + ?
-
?
beet arabinan + H2O Streptomyces purpurascens
-
L-arabinose + ?
-
?

Organism

Organism UniProt Comment Textmining
Albifimbria verrucaria
-
-
-
Aspergillus niger
-
-
-
Athelia rolfsii
-
-
-
Bacillus subtilis
-
-
-
Botrytis cinerea
-
-
-
Botrytis fabae
-
-
-
Clostridium felsineum
-
-
-
Kitasatospora aureofaciens
-
-
-
Kitasatospora aureofaciens KA 204
-
-
-
Pectobacterium carotovorum
-
-
-
Rhodotorula flava
-
-
-
Sclerotinia fructigena
-
-
-
Sclerotinia libertiana
-
-
-
Streptomyces flaveolus
-
-
-
Streptomyces flaveolus KA1143
-
-
-
Streptomyces lavendulae
-
-
-
Streptomyces lavendulae KA140
-
-
-
Streptomyces massasporeus
-
IFO 3841
-
Streptomyces olivochromogenes
-
IFO3178
-
Streptomyces olivochromogenes IFO3178
-
IFO3178
-
Streptomyces purpurascens
-
IFO 3389
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger
-
Streptomyces purpurascens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82.9
-
-
Streptomyces purpurascens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beet arabinan + H2O
-
Bacillus subtilis L-arabinose + ?
-
?
beet arabinan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
beet arabinan + H2O
-
Pectobacterium carotovorum L-arabinose + ?
-
?
beet arabinan + H2O
-
Athelia rolfsii L-arabinose + ?
-
?
beet arabinan + H2O
-
Rhodotorula flava L-arabinose + ?
-
?
beet arabinan + H2O
-
Streptomyces purpurascens L-arabinose + ?
-
?
L-arabinan + H2O
-
Bacillus subtilis (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces olivochromogenes (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Kitasatospora aureofaciens (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Albifimbria verrucaria (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces lavendulae (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces purpurascens (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces flaveolus (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Botrytis fabae (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces lavendulae KA140 (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces olivochromogenes IFO3178 (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Kitasatospora aureofaciens KA 204 (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces flaveolus KA1143 (1,5-alpha-L-arabinose)25 + ?
-
?
additional information
-
Bacillus subtilis ?
-
?
additional information
-
Aspergillus niger ?
-
?
additional information
-
Pectobacterium carotovorum ?
-
?
additional information
-
Rhodotorula flava ?
-
?
additional information inactive towards arabinan, arabinoxylan and arabinogalactan Streptomyces purpurascens ?
-
?
additional information inactive on p-nitrophenyl beta-D-galactopyranoside, phenyl beta-L-arabinopyranosideand p-nitrophenyl glycosides like alpha-D-galactopyranoside, alpha-and beta-D-glucopyranosides, alpha- and beta-D-xylopyranosides, alpha-L-fucopyranoside, alpha-D-mannopyranoside and N-acetyl beta-D-glucosaminide Streptomyces purpurascens ?
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aspergillus niger p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces purpurascens p-nitrophenol + L-arabinose
-
?

Subunits

Subunits Comment Organism
octamer 8 * 62000 Streptomyces purpurascens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Bacillus subtilis
6.5
-
-
Streptomyces purpurascens