Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
General Stability | Organism |
---|---|
denaturation and unfolding induced with 2 M guanidine hydrochloride pH 7.0 or 25 mM glycine phosphate buffer, pH 2 for 4 h at 25°C, chaperons GroEL, GroES assist in vivo and in vitro folding of the enzyme, a trans folding mechanism for GroEL/GroES-assisted refolding of MalZ is proposed | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-chelating column HisTrap HP chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0424 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltodextrin + H2O | - |
Escherichia coli | maltose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
cyclomaltodextrinase | - |
Escherichia coli |
maltodextrin glucosidase | MalZ | Escherichia coli |