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Literature summary for 3.2.1.54 extracted from

  • Paul, S.; Singh, C.; Mishra, S.; Chaudhuri, T.K.
    The 69 kDa Escherichia coli maltodextrin glucosidase does not get encapsulated underneath GroES and folds through trans mechanism during GroEL/GroES-assisted folding (2007), FASEB J., 21, 2874-2885.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

General Stability

General Stability Organism
denaturation and unfolding induced with 2 M guanidine hydrochloride pH 7.0 or 25 mM glycine phosphate buffer, pH 2 for 4 h at 25°C, chaperons GroEL, GroES assist in vivo and in vitro folding of the enzyme, a trans folding mechanism for GroEL/GroES-assisted refolding of MalZ is proposed Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni-chelating column HisTrap HP chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0424
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltodextrin + H2O
-
Escherichia coli maltose
-
?

Subunits

Subunits Comment Organism
monomer
-
Escherichia coli

Synonyms

Synonyms Comment Organism
cyclomaltodextrinase
-
Escherichia coli
maltodextrin glucosidase MalZ Escherichia coli