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Literature summary for 3.2.1.54 extracted from
- Biochemical and structural features of a novel cyclodextrinase from cow rumen metagenome (2007), Biotechnol. J., 2, 207-213.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | More |
General Stability
| General Stability | Organism |
|---|---|
| stable in the presence of up to 2% Triton X-100, completely inactivated in the presence of SDS at concentrations higher than 50 mM | More |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
More |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slightly activating | More | |
| Mn2+ | slightly activating | More | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 280000 | - |
SDS-PAGE, size exclusion column chromatography | More |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| More | - |
DNA isolated from Bos taurus rumen fluid | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein | More |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80 | - |
potato starch as substrate, maltose, kejibiose, nigeriose, iso-maltose, iso-maltotriose, trehalose and p-nitrophenyl ?-D-glucopyranoside are not used as substrates | More |
| 360 | - |
amylopectin as substrate | More |
| 1640 | - |
pullulan as substrate | More |
| 1680 | - |
amylose n as substrate | More |
| 2600 | - |
soluble starch as substrate | More |
| 3960 | - |
cyclomaltooctaose as substrate | More |
| 4680 | - |
cyclomaltohexaose as substrate | More |
| 4920 | - |
cyclomaltoheptaose as substrate | More |
| 5000 | - |
maltooligosaccharides as substrate | More |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
More | D-glucose + ? | - |
? | |
| amylose + H2O | - |
More | D-glucose + ? | - |
? | |
| cyclomaltodextrin + H2O | - |
More | linear maltodextrin | - |
? | |
| cyclomaltoheptaose + H2O | - |
More | D-glucose | - |
? | |
| cyclomaltohexaose + H2O | - |
More | D-glucose | - |
? | |
| cyclomaltooctaose + H2O | - |
More | D-glucose | - |
? | |
| maltooligosaccharides + H2O | - |
More | D-glucose + ? | - |
? | |
| potato starch + H2O | - |
More | D-glucose | - |
? | |
| pullulan + H2O | - |
More | D-glucose + ? | - |
? | |
| soluble starch + H2O | - |
More | D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4*70000 | More |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDase | - |
More |
| cyclomaltodextrinase | - |
More |
| RA.04 | - |
More |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
More |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | half-life at 60°, 65°, 70° and 80°C is 600, 480, 30 and 12 min, respectively, independent on the pH and the presence of Ca2+ | More |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
More |
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Release 2023.1 (January 2023)
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