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Literature summary for 3.2.1.54 extracted from

  • Kaulpiboon, J.; Pongsawasdi, P.
    Expression of cyclodextrinase gene from Paenibacillus sp. A11 in Escherichia coli and characterization of the purified cyclodextrinase (2004), J. Biochem. Mol. Biol., 37, 408-415.
    View publication on PubMed
Search for more literature for 3.2.1.54

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol recombinant enzyme Paenibacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression by using the pUC 18 vector in Escherichia coli JM 109 Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide 5 mM, complete inhibition Paenibacillus sp.
AgNO3 10 mM, complete inhibition Paenibacillus sp.
BaCl2 10 mM, 12% inhibition Paenibacillus sp.
diethyldicarbonate 1 mM, complete inhibition, recombinant enzyme Paenibacillus sp.
HgCl2 10 mM, complete inhibition Paenibacillus sp.
iodoacetamide 5 mM, 29% inhibition Paenibacillus sp.
MgCl2 10 mM, 20% inhibition Paenibacillus sp.
N-bromosuccinimide 0.1 mM, 95% inhibition, recombinant enzyme Paenibacillus sp.
Phenylglyoxal 5 mM, 38% inhibition Paenibacillus sp.
ZnCl2 10 mM, 63% inhibition Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 beta-cyclodextrin
-
 Paenibacillus sp.
2.9
-
 gamma-cyclodextrin
-
 Paenibacillus sp.
5
-
 alpha-cyclodextrin
-
 Paenibacillus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm recombinant enzyme is produced in Escherichia coli primarily in Paenibacillus sp. 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
-
-
Paenibacillus sp. A11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O 57% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + less amounts of maltotriose and maltotetraose ?
beta-cyclodextrin + H2O
-
 Paenibacillus sp. D-glucose + maltose + less amounts of maltotriose and maltotetraose ?
gamma-cyclodextrin + H2O 72% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + less amounts of maltotriose and maltotetraose ?
maltoheptaose + H2O 43% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + ?
-
 ?
maltoheptaose + H2O 43% of the activity with beta-cyclodextrin Paenibacillus sp. A11 D-glucose + maltose + ?
-
 ?
maltohexaose + H2O 35% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + ?
-
 ?
maltohexaose + H2O 35% of the activity with beta-cyclodextrin Paenibacillus sp. A11 D-glucose + maltose + ?
-
 ?
maltopentaose + H2O 22% of the activity with beta-cyclodextrin Paenibacillus sp. D-glucose + maltose + ?
-
 ?
maltopentaose + H2O 22% of the activity with beta-cyclodextrin Paenibacillus sp. A11 D-glucose + maltose + ?
-
 ?
maltotetraose + H2O 14% of the activity with beta-cyclodextrin Paenibacillus sp. 2 D-glucose + maltose
-
 ?
maltotetraose + H2O 14% of the activity with beta-cyclodextrin Paenibacillus sp. A11 2 D-glucose + maltose
-
 ?
maltotriose + H2O 11% of the activity with beta-cyclodextrin Paenibacillus sp. maltose + D-glucose
-
 ?
maltotriose + H2O 11% of the activity with beta-cyclodextrin Paenibacillus sp. A11 maltose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Paenibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
 alpha-cyclodextrin
-
 Paenibacillus sp.
26.7
-
 gamma-cyclodextrin
-
 Paenibacillus sp.
36.7
-
 beta-cyclodextrin
-
 Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Paenibacillus sp.