Cloned (Comment) | Organism |
---|---|
gene cgtB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) | Thermococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
x * 79000, recombinant enzyme, SDS-PAGE | Thermococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | - |
- |
- |
Thermococcus sp. B1001 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by heat denaturation | Thermococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
640 | - |
purified recombinant enzyme, substrate gamma-cyclomaltodextrin | Thermococcus sp. |
1080 | - |
purified recombinant enzyme, substrate alpha-cyclomaltodextrin | Thermococcus sp. |
1940 | - |
purified recombinant enzyme, substrate beta-cyclomaltodextrin | Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclomaltodextrin + H2O | 55% of the activity with beta-cyclomaltodextrin | Thermococcus sp. | alpha-D-glucose + maltose | - |
? | |
alpha-cyclomaltodextrin + H2O | 55% of the activity with beta-cyclomaltodextrin | Thermococcus sp. B1001 | alpha-D-glucose + maltose | - |
? | |
beta-cyclomaltodextrin + H2O | best substrate | Thermococcus sp. | alpha-D-glucose + maltose | - |
? | |
beta-cyclomaltodextrin + H2O | best substrate | Thermococcus sp. B1001 | alpha-D-glucose + maltose | - |
? | |
gamma-cyclomaltodextrin + H2O | 33% of the activity with beta-cyclomaltodextrin | Thermococcus sp. | alpha-D-glucose + maltose | - |
? | |
gamma-cyclomaltodextrin + H2O | 33% of the activity with beta-cyclomaltodextrin | Thermococcus sp. B1001 | alpha-D-glucose + maltose | - |
? | |
linear maltodextrins + H2O | with a small glucose unit, low activity | Thermococcus sp. | alpha-D-glucose + maltose | - |
? | |
linear maltodextrins + H2O | with a small glucose unit, low activity | Thermococcus sp. B1001 | alpha-D-glucose + maltose | - |
? | |
additional information | no activity with pullulan and maltose, soluble starch is a poor substrate | Thermococcus sp. | ? | - |
? | |
additional information | no activity with pullulan and maltose, soluble starch is a poor substrate | Thermococcus sp. B1001 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, recombinant enzyme, SDS-PAGE | Thermococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
CDase | - |
Thermococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
recombinant enzyme | Thermococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Thermococcus sp. |