Literature summary for 3.2.1.54 extracted from

Fritzsche, H.B.; Schwede, T.; Schulz, G.E.
Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 (2003), Eur. J. Biochem., 270, 2332-2341.

Search for more literature for 3.2.1.54

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, expression as native and selenomethionine derivatives in Eschrichia coli BL21(DE3) and B834 (DE3), respectively	Flavobacterium sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
6 mg/ml purified recombinant enzyme as native and selenomethionine derivatives in 50 mM HEPES, pH 7.5, 3.8 M NaCl, and 3 mM DTT with the SeM-enzyme derivative, equal volumes of protein and reservoir solution, hanging drop vapour diffusion method, 2 days, 15% glycerol for cryoprotection, multiwavelength anomalous X-ray diffraction 3D-structure determination and analysis at 2.1 A resolution	Flavobacterium sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	determination of the binding site	Flavobacterium sp.

Organism

Organism	UniProt	Comment	Textmining
Flavobacterium sp.	Q8KKG0	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli	Flavobacterium sp.

Reaction

Reaction	Comment	Organism	Reaction ID
cyclomaltodextrin + H2O = linear maltodextrin	active site structure	Flavobacterium sp.	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-cyclomaltodextrin + H2O	-	Flavobacterium sp.	alpha-D-glucose + maltose	-	?
beta-cyclomaltodextrin + H2O	-	Flavobacterium sp.	alpha-D-glucose + maltose	-	?
gamma-cyclomaltodextrin + H2O	-	Flavobacterium sp.	alpha-D-glucose + maltose	-	?
pullulan + H2O	-	Flavobacterium sp.	?	-	?
starch + H2O	-	Flavobacterium sp.	alpha-D-glucose + maltose	-	?

Synonyms

Synonyms	Comment	Organism
CD-degrading enzyme	-	Flavobacterium sp.
CDase	-	Flavobacterium sp.
cyclodextrinase	-	Flavobacterium sp.
More	enzyme belongs to the glycosylhydrolase family 13	Flavobacterium sp.

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Release 2023.1 (January 2023)