Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain MC1061 | Bacillus sp. (in: Bacteria) |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified recombinant enzyme in 10 mM HEPES, pH 7.5 and 1 mM 2-mercaptoethanol, hanging drop vapour diffusion method by addition of equal amount of 0.002 ml of precipitant solution containing 50% PEG 200, and 0.1 M HEPES, pH 7.0, equilibration against 1 ml, 22°C, X-ray diffraction structure determination and analysis at 3.3 A resolution | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a mutant enzyme lacking the C-terminal domain, expression as insoluble protein in Escherichia coli | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.43 | - |
beta-cyclomaltodextrin | pH 6.0, dimer | Bacillus sp. (in: Bacteria) | |
8.68 | - |
beta-cyclomaltodextrin | pH 7.0, dodecamer | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
alcalophilic strain I-5 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ammonium sulfate precipitation and Sepharose Q chromatography | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclomaltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | alpha-D-glucose + maltose | - |
? | |
beta-cyclomaltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | alpha-D-glucose + maltose | - |
? | |
gamma-cyclomaltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | alpha-D-glucose + maltose | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) | alpha-D-glucose + maltose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | domain-swapped dimer | Bacillus sp. (in: Bacteria) |
dodecamer | dodecameric assembly of 6 copies of the dimer, most active form | Bacillus sp. (in: Bacteria) |
More | pH-dependent association and dissociation of the enzyme dimeric units, structure determination and analysis, enzyme has a beta-stranded N-terminal domain, residues 1-123, a central (alphabeta)8 barrel domain, and a beta-stranded C-terminal domain, residues 505-583 | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
CDase | - |
Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
beta-cyclomaltodextrin | pH 7.0, dodecamer | Bacillus sp. (in: Bacteria) | |
1.68 | - |
beta-cyclomaltodextrin | pH 6.0, dimer | Bacillus sp. (in: Bacteria) |