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Literature summary for 3.2.1.54 extracted from

  • Yang, S.J.; Wang, Z.; Zhang, S.Z.
    Purification and properties of cyclodextrinase from Bacillus stearothermophilus HY-1 (1996), Ann. N. Y. Acad. Sci., 799, 425-428.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ relative activity 1.6% Geobacillus stearothermophilus
diethyldicarbonate DEPC 10 mM, relative activity 2.3% Geobacillus stearothermophilus
Fe3+ relative activity 33% Geobacillus stearothermophilus
Hg2+ relative activity 1.6% Geobacillus stearothermophilus
N-bromosuccinimide 0.1 mM, relative activity 8%, 1.0 mM, relative activity 4.6% Geobacillus stearothermophilus
Zn2+ relative activity 1.5% Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
HY-1
-
Geobacillus stearothermophilus HY-1
-
HY-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Geobacillus stearothermophilus maltose + ?
-
?
amylopectin + H2O
-
Geobacillus stearothermophilus HY-1 maltose + ?
-
?
amylose + H2O
-
Geobacillus stearothermophilus ?
-
?
amylose + H2O
-
Geobacillus stearothermophilus HY-1 ?
-
?
cyclomaltodextrin + H2O
-
Geobacillus stearothermophilus maltodextrin
-
?
cyclomaltodextrin + H2O
-
Geobacillus stearothermophilus HY-1 maltodextrin
-
?
pullulan + H2O
-
Geobacillus stearothermophilus panose + maltose + glucose
-
?
pullulan + H2O
-
Geobacillus stearothermophilus HY-1 panose + maltose + glucose
-
?
starch + H2O
-
Geobacillus stearothermophilus glucose + maltose
-
?
starch + H2O
-
Geobacillus stearothermophilus HY-1 glucose + maltose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Geobacillus stearothermophilus